Anti-Phospho-ErbB4/HER4-Y1284 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q15303

Core Product Specifications and Parameters

Biological Background: ErbB4/HER4 Function and Localization

• Receptor tyrosine-protein kinase erbB-4 (ErbB4/HER4) is a member of the epidermal growth factor receptor (EGFR/ErbB) family, which also includes EGFR/ErbB1, ErbB2, and ErbB3.
• Acts as a cell surface receptor for neuregulins (NRG1-4) and EGF family members (BTC, EREG, HBEGF), triggering dimerization and autophosphorylation to initiate downstream signaling cascades.
• Regulates critical developmental processes including cardiac muscle differentiation, central nervous system development (neural crest migration and axon guidance), and mammary gland differentiation and lactation.
• Alternative splicing generates functionally distinct isoforms (e.g., JM-A, JM-B, CYT-1, CYT-2) that differ in signaling capabilities, such as PI3K/AKT pathway activation and nuclear translocation.
• Proteolytic processing of JM-A isoforms releases soluble intracellular domains (4ICD) that translocate to the nucleus to regulate transcription (e.g., STAT5A-mediated milk protein expression) or to mitochondria to promote apoptosis.
• Subcellular localization spans the cell membrane, nucleus, and mitochondrion, reflecting its diverse signaling roles.
• Expressed at highest levels in brain, heart, and kidney, with lower levels in thymus, lung, salivary gland, and pancreas; isoform JM-B is predominantly expressed in heart.
• Post-translational modifications include tyrosine phosphorylation, glycosylation, and ubiquitination, with key roles in signal transduction and receptor downregulation.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to residues surrounding Y1284 of human ErbB4; verify specificity using phosphatase-treated controls or non-phosphorylated peptide competition in your assay system.
• For Western blot (WB), anticipate a major band at approximately 147 kDa; optimize blocking and antibody dilution to reduce non-specific binding, especially in complex tissue lysates.
• In immunofluorescence/immunocytochemistry (IF/ICC), include permeabilization steps and validate phospho-signal colocalization with membrane or nuclear markers depending on isoform context.
• For ELISA, consider using a capture/detection pair with a total ErbB4 antibody to normalize phospho-signal and ensure quantitative accuracy.
• Because ErbB4 signaling is context-dependent, validate findings in physiologically relevant cell lines (e.g., MCF-7 for breast, H9c2 for cardiac) with known NRG1 stimulation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, specializing in antibodies, antigens, and ancillary reagents for diagnostic manufacturers and research laboratories. Anti-Phospho-ErbB4/HER4-Y1284 Rabbit mAb supports investigations into ErbB4/HER4 signaling pathways relevant to oncology, neurology, and cardiac development. Through our extensive supply network, CamelBio offers validated antibody pairs, bulk phosphorylated-site-specific antibodies, and custom sourcing for rare targets—streamlining procurement from concept to clinic.