Anti-Phospho-ErbB3/HER3-Y1222 Monoclonal Antibody for WB, ELISA - P21860

Core Product Specifications and Parameters

Biological Background: ErbB3/HER3 Function and Localization

• ErbB3 is a receptor tyrosine-protein kinase that acts as a cell surface receptor for neuregulins. Ligand binding triggers tyrosine phosphorylation and promotes association with the p85 subunit of PI3K. Related references: PMID:20682778
• ErbB3 may also be activated by CSPG5, a chondroitin sulfate proteoglycan. Related references: PMID:15358134
• It plays a role in the regulation of myeloid cell differentiation. Related references: PMID:27416908
• Alternative splicing generates multiple isoforms; common aliases include HER3, FERLK, LCCS2, and c-erbB3.
• Subcellular localization includes the cell membrane and secretion.
• Tissue expression is predominantly in epithelial tissues and the brain.
• Post-translational modifications encompass phosphorylation, glycosylation, and disulfide bond formation, classifying it as a phosphoprotein and glycoprotein.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to Y1222 of human ERBB3. It specifically detects the phosphorylation at this site; confirm specificity by phosphatase treatment or comparison with non-phospho peptide blocking.
• For Western blotting, the predicted molecular weight is approximately 148 kDa. Run appropriate molecular weight markers and use a suitable lysis buffer containing phosphatase inhibitors to preserve phosphorylation.
• In ELISA, the antibody can be used as a capture or detection antibody for phosphorylated ERBB3 peptides or proteins. Validate performance in your sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic manufacturers and researchers with high-quality antibodies and ancillary reagents. For studies on ErbB3/HER3 signaling, we offer validated antibody pairs, optimized monoclonal antibodies, bulk reagents, and rare target sourcing to accelerate your assay development from concept to clinic.