Anti-Phospho-eNOS (S1177) Rabbit Monoclonal Antibody for WB, ELISA - P29474

Core Product Specifications and Parameters

Biological Background: eNOS Function and Localization

• eNOS (Nitric oxide synthase 3) catalyzes the production of nitric oxide (NO), which acts as a vasodilator through cGMP-mediated smooth muscle relaxation. Related references: PMID:1378832
• NO produced by eNOS is also pivotal for VEGF-mediated angiogenesis and platelet activation, linking endothelial function to circulatory health.
• Alternative splicing generates a dominant-negative isoform that can heterodimerize with the full-length enzyme, potentially down-regulating eNOS activity.
• eNOS is localized to caveolae at the plasma membrane, the Golgi apparatus, and the cytoskeleton, allowing compartmentalized NO signaling.
• The protein undergoes multiple post-translational modifications, including phosphorylation at Ser1177 (associated with activation), myristoylation, and palmitoylation, which regulate its subcellular targeting and activity.
• eNOS expression is detected in platelets, placenta, liver, and kidney, with vascular endothelial cells being a primary site of function.
• The enzyme requires multiple cofactors: heme, FAD, FMN, NADPH, and zinc, and binds calmodulin in a calcium-dependent manner for activation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to the Ser1177 region, suggesting that the antibody recognizes the phosphorylated form of eNOS at this site.
• For Western blot, consider using phosphatase inhibitors in lysis buffers to preserve phosphorylation and validate band specificity using phosphatase‑treated controls.
• ELISA applications may require optimization for coating and detection conditions; consider testing a range of antibody dilutions and blocking agents.
• Validate specificity in relevant human cell or tissue lysates, as cross‑reactivity with other species is not indicated; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents. With this anti‑phospho‑eNOS (Ser1177) monoclonal antibody, we support studies on endothelial dysfunction and cardiovascular disease. We also offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development.