Anti-Phospho-eNOS-S1177 Polyclonal Antibody for WB, IHC-P, ELISA - P29474

Core Product Specifications and Parameters

Biological Background: eNOS Function and Localization

• eNOS (Nitric oxide synthase 3) is a constitutively expressed enzyme that catalyzes the production of nitric oxide (NO), a critical signaling molecule implicated in vascular smooth muscle relaxation through a cGMP-mediated pathway and VEGF-induced angiogenesis. Related references: PMID:1378832
• NO produced by eNOS also plays a role in platelet activation and blood clotting regulation.
• The enzyme localizes to the plasma membrane, caveolae, cytoplasm, cytoskeleton, and Golgi apparatus, reflecting its dynamic subcellular regulation.
• eNOS is expressed in platelets, placenta, liver, and kidney.
• Alternative splicing generates a dominant-negative isoform that lacks eNOS activity and may downregulate eNOS function by forming heterodimers with isoform 1.
• Key post-translational modifications include phosphorylation at Ser1177 (the epitope recognized by this antibody), as well as myristoylation and palmitoylation for membrane anchoring.
• eNOS activity depends on cofactors such as heme, FAD, FMN, NADP, calmodulin, and metals like calcium and zinc.

Experimental Guidance and Technical Tips

• For Western blotting, include appropriate controls (e.g., unstimulated versus stimulated endothelial cells) and phosphatase inhibitors to verify phospho-specificity and prevent dephosphorylation.
• In immunohistochemistry (IHC-P), validate the antibody in paraffin-embedded sections using antigen retrieval and perform parallel staining with a phosphorylation-independent eNOS antibody to assess specificity.
• For ELISA applications, the antibody can be used to capture or detect phospho-eNOS (Ser1177) in cell lysates or recombinant protein preparations; consider using a total eNOS antibody for normalization.
• The predicted molecular weight of eNOS is approximately 133 kDa; use appropriate molecular weight markers and gel percentages for clear resolution.
• Cross-reactivity has been reported for human and mouse; if using other species, perform a sequence alignment to assess epitope conservation and empirically validate reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams by providing a single access point for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This phospho-eNOS (Ser1177) antibody is ideal for vascular biology and endothelial signaling studies, and CamelBio can assist with rare target sourcing and custom reagent procurement to streamline your project from concept to clinic.