Anti-Phospho-ELK1-S383 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P19419

Core Product Specifications and Parameters

| Product Name | Phospho-ELK1-S383 Rabbit pAb | | Remarks/Alias | ELK1; Phospho-ELK1-S383 | | Species | Human | | GeneID (Human) | 2002 | | GeneID | 2002 | | Immunogen | A synthetic phosphorylated peptide around S383 of human ELK1 (NP_005220.2). | | Source | Rabbit | | Category | Phosphorylated Antibodies | | Application | WB, IHC-P, ELISA | | Cross Reactivity | Human, Mouse, Rat | | SWISS | P19419 | | Protein Weight | 45kDa | | Shipping | Ice bag |

Biological Background: ELK1 Function and Localization

• ELK1 (ETS domain-containing protein Elk-1) is a transcription factor that binds to purine-rich DNA sequences, mediating gene regulation. Related references: PMID:10799319 PMID:7889942

• It forms a ternary complex with serum response factor (SRF) on the serum response element (SRE) of immediate early genes such as FOS and IER2. Related references: PMID:1630903

• ELK1 is activated through JNK and MAPK signaling pathways, which phosphorylate the protein at sites including S383, leading to target gene transcription. Related references: PMID:7889942

• The protein is predominantly localized in the nucleus, consistent with its role in transcriptional regulation.

• Tissue expression is specific, with notable levels in lung and testis.

• ELK1 undergoes alternative splicing and multiple post-translational modifications, including phosphorylation, ubiquitination (isopeptide bond formation), and glycosylation.

• Additional functional annotations include activator, DNA-binding, phosphoprotein, and Ubl conjugation.

Experimental Guidance and Technical Tips

• For Western blot (WB), validate phospho-specificity by treating samples with lambda protein phosphatase; use phosphatase inhibitors during lysis to preserve phospho-signal.

• In immunohistochemistry-paraffin (IHC-P), consider antigen retrieval with sodium citrate buffer (pH 6.0) and optimize antibody incubation conditions.

• For ELISA, employ appropriate blocking and detection protocols; verify cross-reactivity with mouse and rat samples if needed, as indicated by supplier data.

• Since ELK1 is nuclear, subcellular fractionation or nuclear extracts may enhance detection sensitivity in WB.

• Antibody performance may vary by application; pilot testing in the specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, empowering diagnostic developers with reliable access to critical reagents like this anti-Phospho-ELK1-S383 antibody. We support transcription and cell signaling research areas with a comprehensive portfolio, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or challenging targets. From concept to clinic, CamelBio streamlines your supply chain so you can focus on accelerating assay development.