Anti-Phospho-eIF4EBP1-T70 Polyclonal Antibody for WB, IHC-P, ELISA - Q13541

Core Product Specifications and Parameters

Biological Background: eIF4EBP1 Function and Localization

• eIF4EBP1 (Eukaryotic translation initiation factor 4E-binding protein 1), also known as PHAS-I, is a key repressor of cap-dependent translation initiation.
• It regulates translation by binding to eIF4E; hypophosphorylated eIF4EBP1 strongly binds eIF4E and prevents eIF4F complex assembly, while hyperphosphorylation (e.g., at Thr70) promotes dissociation and allows translation to proceed.
• The phosphorylation status of eIF4EBP1 is controlled by the mTORC1 and MAP kinase signaling pathways in response to hormones, growth factors, and nutrients.
• Subcellular localization includes both cytoplasm and nucleus, suggesting roles in spatial regulation of translation.
• Multiple post-translational modifications besides phosphorylation, such as acetylation and ubiquitination, may modulate its stability and function.
• eIF4EBP1 is implicated in cell growth, proliferation, and metabolism, and its dysregulation is associated with cancers and metabolic disorders.

Experimental Guidance and Technical Tips

• For Western blot, include phospho-specificity controls (e.g., phosphatase treatment or non-phospho peptide blocking) to confirm band specificity.
• Optimal working dilutions should be determined empirically; typical starting ranges for WB are 1:500–1:2000.
• For IHC-P, antigen retrieval and blocking steps are recommended; validate in tissues with known phosphorylated eIF4EBP1 expression.
• Cross-reactivity with mouse and rat is reported; verify conservation of the Thr70 phospho-epitope in species of interest before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Phospho-eIF4EBP1-T70 polyclonal antibody is part of our extensive catalog of validated phospho-specific antibodies supporting translation regulation and mTOR pathway research. Through our B2B platform, you can efficiently source optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development and production.