Anti-Phospho-EIF4B-S422 Polyclonal Antibody for WB, ELISA - P23588

Core Product Specifications and Parameters

Biological Background: EIF4B Function and Localization

• EIF4B is a eukaryotic translation initiation factor required for the binding of mRNA to ribosomes.
• It functions in close association with EIF4F and EIF4A, binding near the 5'-terminal cap of mRNA in the presence of EIF4F and ATP.
• EIF4B promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4A and EIF4F.
• The EIF4B gene undergoes alternative splicing, producing multiple isoforms.
• The protein is subject to post-translational modifications including phosphorylation, acetylation, and ubiquitination (Ubl conjugation).
• Direct protein sequencing and proteomics identification confirm its expression in human cells.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phospho-peptide representing the phosphorylation site at S422; it should specifically detect endogenous EIF4B only when phosphorylated at this residue.
• For Western blot, consider using phosphatase inhibitors during sample preparation to preserve phosphorylation status. Validate specificity by treating cell lysates with λ‑phosphatase or by competition with the non-phosphorylated peptide.
• In ELISA, the phosphorylated peptide may serve as the coating antigen; include appropriate controls using non-phosphorylated peptide to confirm phosphorylation dependence.
• Optimize antibody dilution and incubation conditions in the relevant sample system; cross‑reactivity has only been tested for human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials and technical services. This phospho‑specific EIF4B antibody is part of our offering for translation initiation research and related assay development. Beyond antibodies, we source validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and even rare‑target raw materials — supporting your project from concept to clinic.