Anti-Phospho-eIF2α (S51) Rabbit Polyclonal Antibody for WB, IP, ELISA - P05198

Core Product Specifications and Parameters

Biological Background: Phospho-eIF2α (S51) Function and Localization

• eIF2α is the alpha subunit of the eIF2 complex that forms a ternary complex with GTP and initiator tRNA, facilitating binding to the 40S ribosomal subunit and assembly of the 43S pre‑initiation complex. Related references: PMID:16289705
• eIF2α is a key component of the integrated stress response (ISR); phosphorylation at Ser51 by kinases such as HRI, PKR, PERK, and GCN2 inhibits global cap‑dependent translation while selectively enhancing translation of stress‑responsive mRNAs like ATF4 and QRICH1. Related references: PMID:19131336, PMID:33384352, PMID:38340717
• In response to mitochondrial damage, phosphorylated eIF2α translocates to the mitochondrial surface to promote PRKN‑independent mitophagy. Related references: PMID:38340717
• eIF2α is localized to the cytoplasm, stress granules, cytosol, and mitochondria, reflecting its dynamic roles in translation control and stress adaptation.
• Post‑translational modifications include phosphorylation (Ser51), acetylation, and others; annotation keywords highlight its involvement in translation regulation, RNA‑binding, and stress response.
• Alternative names for eIF2α include eIF‑2‑alpha, eIF‑2A, eIF‑2alpha, and eIF2‑alpha.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser51 of human eIF2α; the antibody is expected to specifically recognize the phosphorylated form. Consider using phosphatase inhibitors during sample preparation to preserve the phospho‑signal.
• Reported applications (WB, IP, ELISA) and cross‑reactivity (human, mouse, rat) suggest suitability for studying stress‑induced eIF2α phosphorylation across common model systems. Optimize dilutions and validate in the relevant sample system.
• For western blotting, the predicted molecular weight is approximately 36 kDa. Verify band specificity using controls such as phosphatase‑treated lysates or siRNA knockdown.

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CamelBio connects diagnostic developers and research labs with premium IVD raw materials, including this high‑quality anti‑phospho‑eIF2α (S51) antibody. Whether you need validated pairs for immunoassays, bulk ancillary reagents, or hard‑to‑source targets for translation regulation and stress‑signaling studies, our streamlined supply network helps accelerate your project from concept to clinic.