Anti-Phospho-EGFR-Y845 Monoclonal Antibody for WB, IF/ICC, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR (Epidermal Growth Factor Receptor), also known as ErbB-1 or HER1, is a transmembrane receptor tyrosine kinase belonging to the ERBB family. An alternatively spliced isoform (isoform 2) may function as an antagonist of EGF action.
• As a receptor tyrosine kinase, EGFR binds various EGF family ligands and triggers intracellular signaling cascades that convert extracellular signals into appropriate cellular responses, including proliferation, differentiation, and survival (PubMed:10805725, PubMed:27153536, PubMed:2790960). Related references: PMID:10805725 PMID:27153536 PMID:2790960
• Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG, and HBEGF/heparin-binding EGF (PubMed:12297049, PubMed:15611079, PubMed:17909029). Related references: PMID:12297049 PMID:15611079 PMID:17909029
• Ligand binding induces receptor homo- and/or heterodimerization and subsequent autophosphorylation on key cytoplasmic tyrosines. Phosphorylated tyrosine 845 (Y845), located in the activation loop of the kinase domain, is critical for stabilizing the active conformation and enhancing catalytic activity.
• Autophosphorylation leads to recruitment of adapter proteins such as GRB2, activating multiple downstream modules including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, STATs, and possibly NF-kappa-B (PubMed:11116146, PubMed:27153536). Related references: PMID:11116146 PMID:27153536
• EGFR directly phosphorylates other proteins, such as RGS16 (activating its GTPase activity) and MUC1 (enhancing its interaction with SRC and beta-catenin) (PubMed:11602604, PubMed:11483589). Related references: PMID:11602604 PMID:11483589
• It positively regulates cell migration via interaction with CCDC88A/GIV, which retains EGFR at the cell membrane upon ligand stimulation (PubMed:20462955). Related references: PMID:20462955
• EGFR localizes primarily to the cell membrane but is also found in the endoplasmic reticulum, Golgi apparatus, nucleus, endosomes, and can be secreted in exosomes. It is ubiquitously expressed, with isoform 2 notably detected in ovarian cancers.
• In addition to its normal physiological roles, EGFR acts as a host cell receptor for hepatitis C virus (HCV) entry, mediating formation of the CD81-CLDN1 coreceptor complex and enhancing viral membrane fusion.

Experimental Guidance and Technical Tips

• Since this antibody recognizes a phosphorylation-dependent epitope, include phosphatase inhibitors in lysis buffers and handle samples quickly at low temperatures to preserve the phosphorylation signal.
• For western blot, expect a major band around 134 kDa. To confirm phospho-specificity, consider treating cell lysates with lambda phosphatase or performing peptide competition assays using the non-phosphorylated counterpart of the immunogen peptide.
• In immunofluorescence/ICC, a predominantly membrane-associated staining pattern is anticipated; co-staining with a plasma membrane marker can help confirm localization.
• For ELISA applications, the antibody may be used for capturing or detecting phosphorylated EGFR; optimize coating and detection conditions empirically.
• Validated for human reactivity; for other species, verify sequence homology around Y845 and cross-reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and essential ancillary reagents. Whether you require this phospho-EGFR-Y845 antibody for cancer signaling research or need custom-sourced materials for rare targets, CamelBio streamlines procurement from concept to clinical application. Contact us to integrate reliable, high-quality reagents into your next project.