Anti-Phospho-EGFR-Y1173 Rabbit Polyclonal Antibody for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that binds EGF family ligands and activates multiple signaling cascades, including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT modules, to regulate cellular responses like proliferation, migration, and survival. Related references: PMID:27153536, PMID:10805725, PMID:2790960
• Upon ligand binding, EGFR undergoes homo- or heterodimerization and autophosphorylation on key cytoplasmic tyrosine residues, including Y1173, creating docking sites for adaptor proteins such as GRB2, which propagate downstream signaling. Related references: PMID:12297049, PMID:15611079
• EGFR can also directly phosphorylate substrates like RGS16 and MUC1, and participates in NF-kappa-B signaling, linking it to G protein-coupled receptor pathways and cell adhesion. Related references: PMID:11602604, PMID:11483589, PMID:11116146
• Isoform 2 of EGFR may function as an antagonist of EGF action and is notably expressed in ovarian cancers.
• EGFR localizes primarily to the cell membrane, but also to endoplasmic reticulum, Golgi, nucleus, endosomes, and can be secreted, reflecting its dynamic trafficking and signaling roles.
• Key post-translational modifications include phosphorylation (on tyrosine, serine, threonine), glycosylation, ubiquitination, and palmitoylation, which regulate its activity, stability, and interaction networks.
• Ubiquitously expressed, EGFR plays roles in development, memory, learning, and pain signaling, and its dysregulation is implicated in various cancers.
• EGFR also serves as a host cell receptor for hepatitis C virus (HCV) entry, facilitating viral internalization into hepatocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide specific to Y1173 of human EGFR. For Western blot, it is recommended to use phospho-specific lysis buffers and phosphatase inhibitors to preserve the phosphorylation state during sample preparation.
• Since EGFR is highly phosphorylated upon ligand stimulation, treating cells with EGF prior to harvest can enhance signal detection in WB; conversely, serum starvation and/or phosphatase treatment may be used to verify phospho-specificity.
• For ELISA, consider using the antibody in a sandwich format with a total EGFR capture antibody to quantify phospho-EGFR levels in cell lysates or tissue extracts.
• Cross-desorption with the non-phosphorylated peptide may be performed to confirm phospho-specificity in both WB and ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibody pairs, custom monoclonal/polyclonal antibodies, and bulk ancillary reagents. This Phospho-EGFR-Y1173 antibody serves as a critical tool for investigations into EGFR signaling pathways, often dysregulated in oncology and infectious disease research. Whether you need optimized detection reagents or assistance sourcing rare-target raw materials, CamelBio bridges the gap from concept to clinic with expert technical and supply chain support.