Anti-Phospho-EGFR-Y1068 Rabbit Monoclonal Antibody for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• Epidermal growth factor receptor (EGFR, ErbB-1) is a member of the ErbB family of receptor tyrosine kinases, characterized by an extracellular ligand-binding domain, a single transmembrane helix, and an intracellular kinase domain.
• EGFR binds multiple ligands of the EGF family, including EGF, TGF-alpha, and HB-EGF, leading to receptor dimerization and autophosphorylation. This activates key signaling pathways such as the RAS-RAF-MEK-ERK, PI3K-AKT, and STATs cascades, thereby regulating cell proliferation, survival, and differentiation. Related references: PMID:10805725, PMID:27153536, PMID:2790960
• Phosphorylated EGFR may activate the NF-kappa-B signaling pathway, contributing to inflammatory and oncogenic processes. Related references: PMID:11116146
• EGFR directly phosphorylates RGS16, enhancing its GTPase activity and potentially linking receptor tyrosine kinase signaling to G protein-coupled receptor pathways. Related references: PMID:11602604
• EGFR phosphorylates MUC1, promoting its association with SRC and beta-catenin, which may influence cell adhesion and oncogenic signaling. Related references: PMID:11483589
• EGFR is predominantly localized at the cell membrane but also traffics to the endoplasmic reticulum, Golgi apparatus, endosomes, and nucleus, enabling diverse signaling modalities.
• EGFR is ubiquitously expressed in human tissues; an isoform 2 is also found in ovarian cancers.
• Post-translational modifications including phosphorylation, glycosylation, ubiquitination, and palmitoylation dynamically regulate EGFR activity and stability (based on UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide targeting residues around Y1068 of human EGFR. This antibody is designed to specifically detect the Y1068-phosphorylated form; for total EGFR measurement, a separate antibody is recommended.
• When performing Western blot, include phosphatase inhibitors during sample preparation to preserve the phosphorylation state, and select cell lines or tissue samples with documented EGFR activation.
• For ELISA, this antibody may serve as a capture or detection reagent for phospho-EGFR. Pairing with a total EGFR antibody can facilitate signal normalization.
• Reported human cross-reactivity suggests optimal performance in human-derived samples; validation in the intended experimental system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop sourcing partner for IVD raw materials, providing comprehensive access to validated antibodies, recombinant proteins, and ancillary reagents from R&D to commercialization. In the field of EGFR signaling and oncology, our portfolio includes phospho-specific antibodies, total antibodies, and customized raw-material solutions to support your assay development. Contact CamelBio to leverage our bulk procurement capabilities, technical consulting, and supply chain expertise for your diagnostic and research needs.