Anti-Phospho-EGFR-S1070 Rabbit Polyclonal Antibody for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase that binds EGF family ligands, triggering receptor dimerization and autophosphorylation, and activates major signaling cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, and STATs modules. Related references: PMID:10805725, PMID:27153536, PMID:2790960
• Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG, and HBEGF/heparin-binding EGF. Related references: PMID:12297049, PMID:15611079, PMID:17909029
• EGFR directly phosphorylates other proteins like RGS16 and MUC1, influencing GTPase activity and cell adhesion, and promotes cell migration via interaction with CCDC88A/GIV. Related references: PMID:11602604, PMID:11483589, PMID:20462955
• Isoform 2 may act as an antagonist of EGF action.
• EGFR plays a role in learning, memory, and pain signaling (By similarity).
• Subcellular localization includes cell membrane, endoplasmic reticulum, Golgi apparatus, nucleus, endosomes, and secreted forms.
• Ubiquitously expressed; isoform 2 is also found in ovarian cancers.
• EGFR acts as a receptor for hepatitis C virus (HCV) entry into hepatocytes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide encompassing S1070 of human EGFR. Consider using peptide competition assays to confirm phospho-specificity.
• For Western blot, treat cells with EGF or other ligands to induce EGFR phosphorylation at S1070; phosphatase treatment can serve as a negative control.
• This antibody may detect a band at ~134 kDa corresponding to full-length EGFR; additional bands may represent degradation products or isoforms.
• ELISA can be used for high-throughput screening of EGFR phosphorylation status.
• Validate in the appropriate human sample system, as cross-reactivity with other species is not specified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to IVD raw materials, technical services, and consulting from concept to clinic. For EGFR/phospho-EGFR research, we supply validated antibody pairs, recombinant proteins, and ancillary reagents. Our portfolio covers phosphorylated targets, membrane receptors, and cancer biomarkers, ensuring you can source everything from antigens to detection antibodies in one place.