Anti-Phospho-Cytokeratin 8 (KRT8)-S74 Rabbit Polyclonal Antibody for WB and ELISA - P05787

Core Product Specifications and Parameters

Biological Background: Cytokeratin 8 (KRT8) Function and Localization

• Keratin 8 (KRT8), also known as cytokeratin-8 (CK-8) and type-II keratin Kb8, is a type II intermediate filament protein encoded by the KRT8 gene in humans.
• Together with its obligate type I partner KRT18, KRT8 forms the keratin intermediate filament network that provides mechanical stability and resilience to simple epithelial cells. Related references: PMID:26823019
• KRT8 is also involved in protecting cells from non-mechanical stress, such as apoptosis.
• It is required for ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber reinforcement. Related references: PMID:26823019
• In striated muscle, KRT8, together with KRT19, may contribute to linking the contractile apparatus to dystrophin at the costameres. Related references: PMID:16000376
• Subcellular localization includes cytoplasm, nucleoplasm, nucleus matrix, and cytoskeleton.
• Post-translational modifications such as phosphorylation, acetylation, methylation, and ubiquitination regulate KRT8 function; this antibody specifically recognizes phosphorylation at Serine 74.
• Tissue expression is observed in simple epithelial cells, including gingival mucosa and hard palate, and in muscle fibers at costameres.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser74; to confirm phospho-specificity, compare signals with and without phosphatase treatment or use non-phosphorylated peptide competition.
• In Western blot, the expected band is at ~54-56 kDa. Validate detection in your sample type and optimize antibody dilution empirically.
• For ELISA, consider using the phosphopeptide as coating antigen and include a non-phosphorylated peptide control.
• Cross-reactivity is reported for human only; if using other species, perform validation due to potential sequence variation.
• Include appropriate loading controls and molecular weight markers for accurate interpretation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare target sourcing. The phospho-KRT8 (S74) antibody supports research into epithelial cell mechanics and intermediate filament regulation. Whatever your diagnostic development requirements, CamelBio streamlines supply from concept to clinic.