Anti-Phospho-CSF-1R (Y708) Rabbit Polyclonal Antibody for WB, ELISA - P07333

Core Product Specifications and Parameters

Biological Background: Phospho-CD115/CSF-1R-Y708 Function and Localization

• CSF1R, encoded by CSF1R (FMS), is a receptor tyrosine kinase that binds CSF1 and IL34, regulating survival, proliferation, and differentiation of monocytes/macrophages.
• Plays a central role in innate immunity and inflammation by promoting pro-inflammatory chemokine release and macrophage function.
• Ligand binding activates key signaling pathways including ERK1/2, JNK, PI3K-AKT, and STAT3/5, leading to phosphorylation of downstream effectors like PIK3R1 and PLCG2. Related references: PMID:20504948 PMID:30982609
• Located at the cell membrane; phosphorylation at Y708 is critical for kinase activation and signal transduction.
• Expressed in bone marrow and blood mononuclear cells; regulates osteoclast differentiation and bone resorption, and is essential for normal bone/tooth development and fertility.
• In the central nervous system, contributes to microglia development. Related references: PMID:30982608
• Post-translationally modified by glycosylation, disulfide bond formation, and ubiquitination; the Y708 site is a key regulatory phosphorylation for downstream signaling.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide corresponding to Y708 of human CSF1R; recommend confirming phospho‑specificity in treated vs. untreated cell models.
• For Western blot, consider including phosphatase inhibitors in lysis buffers to preserve phosphorylation signals, and validate with appropriate controls (e.g., CSF1 stimulation).
• ELISA may be suitable for detecting phosphorylated CSF1R in cell lysates; ensure antibody compatibility with your specific assay format.
• Cross‑reactivity is reported with human; for other species, verify sequence homology around Y708 before use.

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CamelBio offers comprehensive sourcing solutions for IVD raw materials, including this high‑quality phospho‑specific antibody for CSF1R signaling research. Whether you need validated antibody pairs for diagnostic development, bulk ancillary reagents, or assistance with rare target sourcing, our team supports every stage from concept to clinic. Explore our portfolio to accelerate your assay development in immunology, oncology, and bone biology.