Anti-Phospho-CRMP2/DPYSL2-T514 Rabbit Polyclonal Antibody for WB, ELISA - Q16555

Core Product Specifications and Parameters

Biological Background: CRMP2/DPYSL2 Function and Localization

• Dihydropyrimidinase-related protein 2 (CRMP2/DPYSL2), also known as collapsin response mediator protein 2, N2A3, or ULIP-2, is a member of the dihydropyrimidinase-related protein family essential for semaphorin signaling.
• It plays a critical role in neuronal development and polarity, axon growth and guidance, and mediates growth cone collapse and cell migration.
• CRMP2 is involved in cytoskeleton remodeling downstream of class 3 semaphorins and may participate in endocytosis.
• The protein localizes to the cytoplasm, cytosol, cytoskeleton, and membrane compartments.
• CRMP2 is ubiquitously expressed across human tissues.
• Post-translational modifications, including phosphorylation at T514, methylation, and S-nitrosylation, regulate its activity, interactions, and cellular functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide around T514; thus the antibody is specific to the phosphorylated form. When performing WB, consider including a dephosphorylated control (e.g., phosphatase-treated lysate) to confirm phosphorylation-specific detection.
• For ELISA, the synthetic phospho-peptide immunogen can be used as a coating antigen to evaluate antibody sensitivity and specificity.
• The antibody cross-reacts with mouse and rat; validate its reactivity in your specific model system before quantitative analyses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner offering comprehensive IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For research on neuronal development, axon guidance, and semaphorin signaling, this anti-phospho-CRMP2/DPYSL2-T514 antibody provides a specific tool for detecting phosphorylated T514. Explore our portfolio to accelerate your diagnostic development and life science research workflows.