Anti-Phospho-CRKL (Y207) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P46109

Core Product Specifications and Parameters

Biological Background: CRKL Function and Localization

• CRKL (Crk-like protein) is an adaptor protein containing SH2 and SH3 domains, which facilitate protein-protein interactions in signaling cascades.
• It is implicated in mediating intracellular signal transduction, acting downstream of various receptor tyrosine kinases.
• The protein is a member of the Crk family of adaptor proteins, which play critical roles in cell proliferation, differentiation, and migration.
• Post-translational modifications include phosphorylation; tyrosine 207 (Y207) is a known regulatory site targeted by this antibody.
• The calculated molecular weight is approximately 34 kDa, consistent with UniProt (33.8 kDa).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide surrounding Y207; for cross‑species applications (e.g., mouse, rat), align target sequences to verify epitope conservation.
• For Western blot, a specific band near 34 kDa is expected; include phosphatase inhibitors during sample preparation and use a non‑phospho peptide block to confirm phospho‑specificity.
• In IHC‑P, antigen retrieval conditions may influence phospho‑epitope integrity — consider gentle retrieval and inclusion of phosphatase inhibitors in blocking buffers.
• For ELISA, pairing with a total CRKL antibody allows normalization of phosphorylation levels; validate linearity and specificity in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive range of IVD raw materials for every stage from concept to clinic. For CRKL‑related signal transduction studies, we supply this validated anti‑Phospho‑CRKL‑Y207 rabbit polyclonal antibody alongside optimized total CRKL antibodies, recombinant proteins, and ancillary reagents. Our team can assist with bulk orders, custom immunogen design, and rare‑target sourcing to streamline your assay development.