Anti-Phospho-CRK/CRKII (Y221) Rabbit Polyclonal Antibody for WB and ELISA - P46108

Core Product Specifications and Parameters

Biological Background: CRK Function and Localization

• CRK is an adapter molecule that functions as a proto-oncogene, transmitting signals from tyrosine kinases to downstream effectors via its SH2 and SH3 domains.
• It plays a key role in cell adhesion, spreading, and migration by mediating BCAR1-CRK-RAPGEF1 signaling and activating RAP1. Related references: PMID:31311869
• CRK is involved in membrane ruffling and cell motility through Rac-dependent pathways, and regulates attachment-induced MAPK8 activation.
• It participates in phagocytosis of apoptotic cells and cell motility by interacting with DOCK1 and DOCK4. Related references: PMID:19004829
• CRK may also regulate EFNA5-EPHA3 signaling, contributing to neuronal development and cell repulsion.
• The protein localizes primarily in the cytoplasm and at the cell membrane, consistent with its role in signal transduction at adhesion sites.
• Post-translational modifications include phosphorylation at tyrosine residues (such as Y221), acetylation, and alternative splicing, which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Y221; ensure phospho-specificity by comparing signals in stimulated vs. unstimulated cell lysates or using phosphatase treatment.
• For WB, consider using a loading control (e.g., total CRK antibody) to normalize for protein expression levels.
• Validate antibody reactivity in your specific sample system (human, mouse, or rat) as cross-reactivity may vary based on sequence conservation.
• Since the predicted molecular weight is ~34 kDa, run appropriate molecular weight markers and verify the band size.
• For ELISA, optimize coating and detection conditions with the peptide antigen to confirm sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For studies on CRK-mediated cell adhesion and migration signaling, we offer validated phospho-specific antibodies like this one, along with matched antibody pairs, recombinant proteins, and bulk ancillary reagents. Our team also assists in sourcing rare-target raw materials to accelerate your kit development.