Anti-Phospho-CRK/CRKII (Tyr221) Rabbit Monoclonal Antibody for WB, ELISA - P46108

Core Product Specifications and Parameters

Biological Background: Adapter molecule crk Function and Localization

• Adapter molecule crk (also known as Proto-oncogene c-Crk; p38) is encoded by the CRK gene and functions as an SH2/SH3 domain-containing adaptor in signal transduction.
• CRK mediates cell branching and adhesion via BCAR1-CRK-RAPGEF1 signaling, which leads to RAP1 activation.
• It regulates cell adhesion, spreading, and migration. Related references: PMID:31311869
• CRK mediates attachment-induced MAPK8 activation, membrane ruffling, and cell motility in a Rac-dependent manner.
• It is involved in phagocytosis of apoptotic cells and cell motility through interactions with DOCK1 and DOCK4. Related references: PMID:19004829
• CRK may regulate EFNA5-EPHA3 signaling (By similarity).
• Subcellular localization: Primarily cytoplasmic, with membrane association upon activation.
• Keywords: Contains SH2 and SH3 domains; subject to acetylation, alternative splicing, and phosphorylation; classified as a proto-oncogene.
• The full-length protein has a calculated molecular weight of 33.8 kDa (observed at ~34 kDa by SDS-PAGE).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Tyr221 of human CRK/CRKII, ensuring high specificity for the phosphorylation site. Include phosphatase inhibitors in lysis buffers to preserve the phosphorylation signal.
• For Western blot, a band at approximately 34 kDa is expected. Consider using phospho-specific antibody protocols and validate in relevant human, mouse, or rat cell lines with known CRK expression.
• ELISA applications may require careful optimization of coating antigen and detection conditions; include appropriate phosphorylated and non-phosphorylated peptide controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This phospho-specific CRK antibody supports research into cell adhesion, migration, and integrin signaling pathways. Explore our comprehensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.