Anti-Phospho-CREB1 (Ser129) Rabbit Polyclonal Antibody for WB, ELISA - P16220

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• CREB1 (Cyclic AMP-responsive element-binding protein 1) is a 35 kDa phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters.
• Transcription activation is enhanced by the TORC coactivators, which act independently of Ser-119 phosphorylation (PubMed:14536081). Related references: PMID:14536081
• CREB1 is involved in diverse cellular processes, including synchronization of circadian rhythmicity and differentiation of adipose cells (By similarity).
• In cardiomyocytes, it regulates the expression of apoptotic and inflammatory response factors in response to ERFE-mediated activation of AKT signaling (By similarity).
• The protein localizes to the nucleus.
• CREB1 undergoes alternative splicing, chromosomal rearrangement, and is subject to phosphorylation, ubiquitination, and other post-translational modifications. It is also involved in host-virus interactions.

Experimental Guidance and Technical Tips

• For Western blotting, consider using phosphatase inhibitors in lysis buffers to preserve the phosphorylation signal at Ser129.
• Verify specificity by peptide competition assays using the phosphorylated and non-phosphorylated peptides around S129, or by treating samples with phosphatases.
• Since CREB1 is activated by various signaling pathways (e.g., cAMP, ERK), validate the antibody’s performance under stimulated and unstimulated conditions in the relevant sample system.
• The antibody is suitable for ELISA; optimize coating and detection conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-phospho-CREB1 (Ser129) antibody supports research into transcription regulation, signal transduction, and circadian biology. For validated antibody pairs, bulk reagents, or rare target sourcing, contact CamelBio to streamline your development.