Anti-Phospho-CREB1-S133 Rabbit pAb for WB, IHC-P, ELISA - P16220

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• CREB1 (Cyclic AMP-responsive element-binding protein 1), also known as CREB or CREB-1, is a transcription factor encoded by the CREB1 gene in humans. Multiple isoforms are produced via alternative splicing.
• It functions as a phosphorylation-dependent transcriptional activator by binding to the cAMP response element (CRE) in promoter regions of target genes. Transcriptional activity is enhanced by TORC coactivators independently of Ser-119 phosphorylation. Related references: PMID:14536081
• CREB1 plays key roles in diverse cellular processes, including the synchronization of circadian rhythms and the differentiation of adipose cells.
• In cardiomyocytes, CREB1 regulates the expression of apoptotic and inflammatory response genes downstream of ERFE-mediated AKT signaling.
• Subcellular localization is predominantly nuclear, consistent with its function as a transcription factor.
• Post-translational modifications are critical for CREB1 regulation: it is a phosphoprotein with phosphorylation at Ser119 (and other sites) modulating activity. This antibody specifically detects phosphorylation at Ser133, a key activation site.
• Additional keywords associated with CREB1 include host-virus interaction, ubiquitin conjugation, and involvement in chromosomal rearrangements, highlighting its multifaceted roles in health and disease.

Experimental Guidance and Technical Tips

• Given the immunogen design (a synthetic phosphorylated peptide around Ser133 of human CREB1), this antibody is expected to specifically recognize the phosphorylated form of CREB1 at Ser133. Validate specificity by comparing signals from phosphatase-treated and untreated samples in your experimental system.
• For Western blot (WB), a band at approximately 35 kDa is anticipated. Optimize blocking and antibody dilution conditions to achieve clean detection.
• In immunohistochemistry (IHC-P), consider using antigen retrieval methods compatible with phospho-specific antibodies, and include appropriate controls to confirm phosphorylation-dependent staining.
• The antibody cross-reacts with mouse and rat CREB1, making it suitable for studies in common model organisms; however, sequence alignment around the phosphorylation site is recommended to confirm epitope conservation.
• ELISA applications may require optimization of coating antigen and detection conditions; a phospho-peptide versus non-phospho-peptide ELISA can help assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-phospho-CREB1 (Ser133) antibody supports research into transcription regulation, circadian biology, and signal transduction pathways. In addition to this product, we offer a comprehensive portfolio of validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to accelerate your assay development and manufacturing workflows.