Anti-Phospho-CREB1-S133 Rabbit Monoclonal Antibody for IHC-P, ELISA - P16220

Core Product Specifications and Parameters

Biological Background: CREB1 Function and Localization

• Encodes cyclic AMP-responsive element-binding protein 1 (CREB1), a member of the basic leucine zipper (bZIP) family of transcription factors.
• Functions as a phosphorylation-dependent transcription activator by binding to cAMP response element (CRE) sequences in target gene promoters.
• Transcription activation is enhanced by TORC coactivators (CRTC1/2/3), independent of phosphorylation at Ser-119. Related references: PMID:14536081
• Regulates key cellular processes including circadian rhythm synchronization and adipocyte differentiation.
• Modulates expression of apoptotic and inflammatory response genes in cardiomyocytes via ERFE-mediated AKT signaling (by similarity).
• Localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• Subject to alternative splicing, phosphorylation (e.g., at Ser133), ubiquitination, and isopeptide bond formation; involved in host-virus interactions and chromosomal rearrangements.
• Predicted molecular weight of 35 kDa (UniProt: 35,136 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser133 of human CREB1. For IHC-P, include appropriate phosphorylation-specific controls (e.g., phosphatase treatment) to confirm signal specificity.
• For ELISA applications, consider pairing this phospho-specific antibody with a total CREB1 antibody to quantify phosphorylation stoichiometry in cellular extracts.
• This antibody is predicted to cross-react with CREB1 from human, mouse, and rat; however, validation in the relevant sample system is recommended.
• Avoid over-fixation or prolonged antigen retrieval that may interfere with phosphorylated epitope recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, including validated antibody pairs, recombinant antigens, and ancillary reagents. This anti-phospho-CREB1 (Ser133) antibody supports transcription and signal transduction research, and we offer related products to accelerate assay development. Contact us for bulk ordering, custom sourcing, or technical consultation to meet your specific project needs.