Anti-Phospho-Chk2-T68 Rabbit Polyclonal Antibody for WB, ELISA - O96017

Core Product Specifications and Parameters

Biological Background: Chk2 Function and Localization

• Serine/threonine-protein kinase Chk2 (CHEK2, CDS1, RAD53 homolog) plays a critical role in checkpoint-mediated cell cycle arrest, DNA repair, and apoptosis in response to DNA double-strand breaks.
• Acts as a tumor suppressor; also negatively regulates cell cycle progression during unperturbed cycles and may contribute to chromosomal instability when absent.
• Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T].
  Related references: PMID:37943659
• Regulates cell cycle checkpoint arrest by phosphorylating and inhibiting CDC25A, CDC25B, and CDC25C phosphatases, leading to inhibitory tyrosine phosphorylation of CDK-cyclin complexes.
• Promotes DNA repair through phosphorylation of BRCA2 (enhancing RAD51 chromatin association) and by stimulating DNA repair gene transcription via FOXM1.
• Mediates apoptosis through phosphorylation of p53/TP53 at Ser-20, MDM4, and PML, as well as through E2F1-dependent transcription of pro-apoptotic genes.
• Under oxidative stress, phosphorylates TRIM32 at Ser-55 to promote ATG7 ubiquitination and autophagy assembly.
  Related references: PMID:37943659
• Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition.
  Related references: PMID:25361978
• Predominantly localizes to the nucleus (nucleoplasm, PML bodies); highly expressed in testis, spleen, colon, and peripheral blood leukocytes, with lower expression in other tissues.
• Contains kinase, ATP-binding and nucleotide-binding domains; classified as a phosphoprotein with roles in mitosis, transcription regulation, and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around T68, suggesting specificity for the phosphorylated form; use appropriate phosphatase inhibitors during sample preparation to preserve phospho-signals.
• For Western blot, consider stimulating cells with DNA-damaging agents (e.g., etoposide, irradiation) to induce Chk2 phosphorylation before lysate preparation.
• Validate the antibody in human samples; background band(s) may appear, and it is advisable to optimize loading and detection conditions.
• For ELISA, the phosphorylated peptide can serve as a coating antigen to confirm specificity; negative controls using non-phosphorylated peptide are recommended.
• An expected band near 61 kDa should be observed in WB; additional bands may reflect splice variants or post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the sourcing of IVD raw materials from concept to clinic, offering diagnostic manufacturers and labs rapid access to this anti-phospho-Chk2 antibody for DNA damage and checkpoint kinase research. Beyond individual clones, our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, ancillary bulk reagents, and rare target sourcing—empowering your assay development with flexible, integrated supply solutions.