Anti-Phospho-Chk1-S345 Rabbit Polyclonal Antibody for WB and ELISA - O14757

Core Product Specifications and Parameters

Biological Background: Chk1 Function and Localization

• Chk1 is a serine/threonine-protein kinase essential for the DNA damage checkpoint, required for cell cycle arrest and activation of DNA repair in response to DNA damage or unreplicated DNA. Related references: PMID:11535615, PMID:12399544, PMID:12446774
• It recognizes the substrate consensus motif [R-X-X-S/T] and phosphorylates CDC25A, CDC25B, and CDC25C, leading to their inhibition via 14-3-3 binding and proteasomal degradation, thereby blocking CDK-cyclin activity and halting cell cycle progression. Related references: PMID:9278511, PMID:12676583, PMID:12676925, PMID:12759351
• Chk1 undergoes phosphorylation at several residues, with Ser-345 being a key site for its activation in response to DNA damage, mediating its kinase activity and checkpoint functions.
• Chk1 phosphorylates RAD51 at Thr-309 to promote homologous recombination repair. Related references: PMID:15665856
• It phosphorylates TP53 on multiple sites, enhancing its stabilization and transcriptional activity to induce cell cycle arrest. Related references: PMID:10673501, PMID:15659650, PMID:16511572
• Chk1 localizes to the nucleus, chromosomes, and centrosomes, reflecting its roles in chromatin-associated processes and mitotic regulation.
• It is ubiquitously expressed, with highest levels in thymus, testis, small intestine, and colon.
• Alternative splicing of the CHEK1 gene produces an endogenous repressor isoform that facilitates the S to G2/M phase transition.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic phosphopeptide corresponding to the phosphorylated Ser-345 residue of human Chk1. This ensures specificity for the activated, phosphorylated form of the kinase.
• For Western blot, consider including phosphatase inhibitors during sample preparation to preserve the phosphorylation signal. Validate the phospho-specificity by treating samples with lambda phosphatase and observing loss of signal.
• The antibody has been tested for reactivity with human, mouse, and rat samples; cross-reactivity with other species should be validated individually.
• In ELISA, the antibody may be suitable for detecting phospho-Chk1 in cell lysates or peptide-based assays. Optimize coating and detection conditions for your specific setup.
• Given Chk1's critical role in DNA damage response, this antibody is a useful tool for studying checkpoint signaling, cell cycle regulation, and cancer biology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD developers, offering validated antibodies like this phospho-Chk1-S345 pAb alongside monoclonal/polyclonal antibodies, ancillary reagents, and tailored raw-material procurement. Whether you need bulk supplies or rare targets for DNA damage and cell cycle research, we bridge the gap from discovery to diagnostic application.