Anti-Phospho-Chk1-S296 Rabbit Monoclonal Antibody for WB, ELISA - O14757

Core Product Specifications and Parameters

Biological Background: CHK1 Function and Localization

• CHK1 (Checkpoint kinase-1) is a serine/threonine-protein kinase encoded by the CHEK1 gene, existing in multiple isoforms via alternative splicing.
• It is essential for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to DNA damage or unreplicated DNA, thereby preserving genomic integrity. Related references: PMID:11535615, PMID:12399544, PMID:12446774
• CHK1 phosphorylates CDC25 family phosphatases (CDC25A, B, C) leading to their inhibition and subsequent CDK-cyclin complex inactivation, which blocks cell cycle progression. Related references: PMID:12676583, PMID:12676925, PMID:12759351, PMID:9278511
• It directly phosphorylates TP53 at multiple C-terminal sites, promoting TP53 activation, cell cycle arrest and suppression of cellular proliferation. Related references: PMID:10673501, PMID:15659650, PMID:16511572
• CHK1 modulates homologous recombination repair by phosphorylating RAD51 at Thr-309, enhancing RAD51 chromatin association and DNA repair. Related references: PMID:15665856
• Additional substrates include FANCE (crosslink repair), TLK1 (chromatin assembly), SPRTN (chromatin recruitment), PABIR1/FAM122A (G2/M checkpoint), and histones (transcriptional regulation), highlighting its multifaceted role in genome maintenance. Related references: PMID:17296736, PMID:12660173, PMID:12955071, PMID:31316063, PMID:33108758, PMID:17380128
• Subcellularly, CHK1 localizes to the nucleus, chromosomes, cytoplasm, and centrosome, consistent with its diverse functions in DNA damage signaling and cell cycle control.
• CHK1 is ubiquitously expressed, with highest levels in thymus, testis, small intestine, and colon, and its activity is tightly regulated by phosphorylation at multiple residues.

Experimental Guidance and Technical Tips

• For Western Blot, include phosphatase inhibitors in lysis buffers to maintain phosphorylation and validate band size near 54 kDa.
• Test antibody on lysates from cells treated with DNA-damaging agents (e.g., etoposide, hydroxyurea) to confirm phospho-specificity at Ser296.
• This antibody is validated for human Chk1; cross-reactivity with other species should be assessed individually.
• For ELISA, consider coating both phosphorylated and non-phosphorylated peptides to demonstrate phospho-selectivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop source for IVD raw materials, offering validated antibodies, bulk reagents, and custom sourcing for rare targets. Our expertise in cell cycle and DNA damage research streamlines your development from discovery to diagnostic application. Partner with us for reliable, high-quality raw materials and technical consulting tailored to your projects.