Anti-Phospho-Chk1-S280 Rabbit Monoclonal Antibody for WB, ELISA - O14757

Core Product Specifications and Parameters

Biological Background: Chk1 Function and Localization

• Serine/threonine-protein kinase Chk1 (CHEK1), also known as checkpoint kinase-1, is a key regulator of cell cycle checkpoints and DNA damage response (PubMed:11535615, PubMed:12399544, PubMed:12446774). Related references: PMID:11535615, PMID:12399544, PMID:12446774
• Functions by phosphorylating CDC25 family phosphatases (CDC25A, CDC25B, CDC25C) on specific serine residues, leading to their inhibition via 14-3-3 binding or proteasomal degradation, thereby preventing CDK activation and arresting cell cycle progression (PubMed:9278511, PubMed:12676583, PubMed:12676925, PubMed:12759351). Related references: PMID:9278511, PMID:12676583, PMID:12676925, PMID:12759351
• Phosphorylates TP53 on multiple C-terminal sites, promoting TP53 acetylation and activation, which contributes to cell cycle arrest and apoptosis (PubMed:10673501, PubMed:15659650, PubMed:16511572). Related references: PMID:10673501, PMID:15659650, PMID:16511572
• Involved in DNA repair by homologous recombination through phosphorylation of RAD51 at Thr-309, facilitating RAD51 chromatin loading, and by regulating FANCE in cross-link repair (PubMed:15665856, PubMed:17296736). Related references: PMID:15665856, PMID:17296736
• Localizes primarily to the nucleus and chromosomes, but also found in the cytoplasm and at centrosomes, reflecting its multifaceted roles in genome maintenance.
• Ubiquitously expressed, with highest levels in thymus, testis, small intestine, and colon.
• Contains an ATP-binding kinase domain and is regulated by phosphorylation; classified under serine/threonine-protein kinases and involved in DNA damage and repair pathways.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S280 of human Chk1; confirm antibody specificity by phosphatase treatment or competitive inhibition with non-phosphorylated peptide.
• This rabbit monoclonal antibody is validated for WB and ELISA; consider using appropriate positive controls (e.g., human cell lines treated with DNA-damaging agents).
• For WB, include phosphatase inhibitors in the lysis buffer to preserve phosphorylation; validate in the relevant sample system.
• The predicted protein weight is 54 kDa; optimize gel percentage and transfer conditions accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, enabling seamless progression from concept to clinic. This anti-Phospho-Chk1-S280 antibody supports cancer and cell cycle research, and is part of our comprehensive portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Partner with CamelBio to streamline your assay development with reliable, research-use materials.