Anti-Phospho-Chk1 (S280) Monoclonal Antibody for WB, ELISA - O14757

Core Product Specifications and Parameters

Biological Background: Serine/threonine-protein kinase Chk1 Function and Localization

• Serine/threonine-protein kinase Chk1 (CHEK1) is a critical kinase for checkpoint-mediated cell cycle arrest and DNA repair. An alternatively spliced isoform (isoform 2) acts as an endogenous repressor of isoform 1, antagonizing its function.
• Required for the DNA damage response: recognizes the substrate consensus sequence [R-X-X-S/T] and phosphorylates CDC25A, CDC25B, and CDC25C, creating 14-3-3 binding sites that inhibit these phosphatases and/or promote CDC25A degradation, resulting in inhibitory tyrosine phosphorylation of CDK-cyclin complexes and cell cycle arrest. Related references: PMID:12676583 PMID:12676925 PMID:12759351 PMID:9278511
• Phosphorylates RAD51 at Thr-309, promoting its release from BRCA2 and enhancing chromatin association, thereby facilitating DNA repair by homologous recombination. Related references: PMID:15665856
• Phosphorylates multiple C-terminal sites of TP53, enhancing its acetylation and activation to promote cell cycle arrest and suppress proliferation. Related references: PMID:10673501 PMID:15659650 PMID:16511572
• Also phosphorylates other substrates, including FANCE (repair of DNA cross-links), TLK1 (chromatin assembly), histone H3.1 at Thr-11 (epigenetic gene silencing), RB1 (promoting E2F interaction), SPRTN (chromatin recruitment), and PABIR1/FAM122A (G2/M checkpoint regulation). Related references: PMID:17296736 PMID:12660173 PMID:12955071 PMID:17380128 PMID:31316063 PMID:33108758
• Subcellular localization includes the nucleus, chromosomes, cytoplasm, and centrosomes, consistent with its roles in checkpoint signaling, DNA repair, and cell cycle regulation.
• Expressed ubiquitously, with highest levels in thymus, testis, small intestine, and colon.

Experimental Guidance and Technical Tips

• The immunogen is a phospho-peptide surrounding Ser280 of human Chk1. The antibody is designed to recognize the phosphorylated form specifically. Validate specificity by comparing signals in phosphatase-treated vs. untreated samples or using a non-phosphorylated blocking peptide.
• For Western blot, using appropriate phosphatase inhibitors during sample preparation is recommended to preserve the phosphorylation signal. A band near 54 kDa is expected for Chk1.
• In ELISA, consider using a plate-bound phospho-peptide and a non-phospho peptide control to confirm phospho-specificity.
• Chk1 phosphorylation at Ser280 is induced by DNA damage. Stimulating cells with DNA-damaging agents (e.g., UV, hydroxyurea) may enhance detection in relevant cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-Phospho-Chk1 (S280) antibody addresses key needs in cell cycle and DNA damage research, and CamelBio offers a full portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to streamline your procurement and accelerate your development of next-generation assays.