Anti-Phospho-CFL1-Y139 Rabbit Polyclonal Antibody for IHC-P, ELISA - P23528

Core Product Specifications and Parameters

Biological Background: Cofilin-1 Function and Localization

• Cofilin-1 (CFL1) is an ~18-19 kDa actin-binding protein that regulates actin dynamics by depolymerizing F-actin in a pH-sensitive manner. Related references: PMID:11812157 PMID:33670794
• As part of the subcortical maternal complex (SCMC), cofilin-1 is essential for early embryonic cell divisions through actin regulation. Related references: PMID:15580268
• It contributes to cell morphology and cytoskeletal organization in epithelial cells. Related references: PMID:21834987
• Cofilin-1 facilitates the trafficking of atypical chemokine receptor ACKR2 to the cell membrane, enhancing chemokine clearance. Related references: PMID:23633677
• The protein localizes to the cytoplasm, cytoskeleton, nucleus matrix, and various cell projections including lamellipodia, ruffles, growth cones, and axons.
• Post-translational modifications include phosphorylation (e.g., at Y139) and acetylation, which may regulate its activity and interactions.
• Widely expressed across tissues, cofilin-1 is a conserved phosphoprotein involved in host-virus interactions and ubiquitination pathways.

Experimental Guidance and Technical Tips

• For IHC-P, consider antigen retrieval methods and blocking conditions that preserve phospho-epitope integrity; validate with phosphatase-treated control samples.
• In ELISA, use the phosphorylated peptide as capture antigen and confirm specificity by comparing reactivity against non-phosphorylated peptide.
• Verify cross-reactivity in mouse/rat tissues by aligning target sequence homology; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, including this phosphorylated cofilin-1 antibody for actin dynamics and signaling research. We support diagnostic manufacturers and laboratories with bulk sourcing of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and rare-target reagents, streamlining your journey from concept to clinic.