Anti-Phospho-CDK9 (T186) Rabbit Polyclonal Antibody for WB, ELISA - P50750

Core Product Specifications and Parameters

Biological Background: CDK9 Function and Localization

• CDK9 is the catalytic subunit of positive transcription elongation factor b (P-TEFb), which phosphorylates the C-terminal domain (CTD) of RNA polymerase II to drive productive transcription elongation. Related references: PMID:10574912, PMID:10757782, PMID:11145967

• It phosphorylates a broad range of substrates, including DSIF, NELF, MYOD1, AR, and KAT5, thereby regulating processes such as transcriptional elongation, muscle differentiation, and histone acetylation. Related references: PMID:10912001, PMID:11112772, PMID:12037670, PMID:29335245, PMID:9857195

• CDK9 is essential for cytokine-inducible transcription networks, facilitating promoter recognition of transcription factors such as RELA/p65 in TNF signaling and STAT3 in IL-6 signaling. Related references: PMID:17956865, PMID:18362169

• P-TEFb coordinates cotranscriptional histone modifications (H2Bub1, H3K4me3, H3K36me3) and mRNA processing, integrating transcription elongation with chromatin dynamics and RNA maturation. Related references: PMID:15564463, PMID:19575011, PMID:19844166

• CDK9 contributes to genome integrity maintenance: the CDK9/cyclin-K complex aids recovery from replication stress, while isoform 2 interacts with KU70/XRCC6 to support DNA repair. Related references: PMID:20493174

• The protein localizes to the nucleus, cytoplasm, and PML bodies, and is ubiquitously expressed.

• CDK9 is regulated by phosphorylation (including at T186) and acetylation, and plays a key role in viral replication, serving as the catalytic subunit of the Tat-associated kinase complex during HIV transcription. Related references: PMID:10393184, PMID:11112772

Experimental Guidance and Technical Tips

• Validate phospho-specificity by comparing signals from untreated and lambda phosphatase-treated lysates in WB.
• The immunogen is a phosphopeptide; the antibody may cross-react with non-phosphorylated CDK9—confirm with appropriate controls.
• For WB, the predicted molecular weight is 43 kDa; use prestained markers covering this range and optimize blocking and antibody dilution conditions.
• In ELISA, ensure the coating antigen exposes the T186 phosphorylation motif; consider using phospho-peptide competitors to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials, from validated antibody pairs and recombinant proteins to ancillary reagents and rare-target sourcing. The Phospho-CDK9-T186 antibody supports studies in transcription elongation, kinase signaling, and cell cycle regulation. For customized or difficult-to-source raw materials, CamelBio delivers expert technical consulting and end-to-end supply solutions to accelerate your assay development.