Anti-Phospho-CDK2-T160 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24941

Core Product Specifications and Parameters

Biological Background: CDK2 Function and Localization

• CDK2 (Cyclin-dependent kinase 2) is a serine/threonine-protein kinase that plays a central role in cell cycle regulation, particularly at the G1-S transition and S phase progression. It phosphorylates critical substrates such as RB1, p53/TP53, NPM1, and BRCA2 to promote DNA synthesis, centrosome duplication, and transcriptional programs. CDK2 is essential for meiosis but dispensable for mitosis. Related references: PMID:10499802 PMID:10884347 PMID:10995386 PMID:11051553

• The kinase activity of CDK2 is tightly controlled by cyclin binding and phosphorylation. Cyclin E/CDK2 complexes drive the G1-S transition, while cyclin A/CDK2 complexes propel S-G2 progression. Full activation requires phosphorylation at Thr-160 within the T-loop by the CDK-activating kinase (CAK) complex. Related references: PMID:18372919 PMID:19238148 PMID:19561645

• CDK2 localizes to multiple subcellular compartments, including the nucleus, centrosome, Cajal body, cytoplasm, cytoskeleton, and endosome, reflecting its diverse functions in cell cycle coordination, DNA repair, and signaling.

• CDK2 participates in DNA damage response and repair pathways. It phosphorylates BRCA2 to regulate homologous recombination repair and phosphorylates NBN to influence telomere maintenance, thereby contributing to genomic stability. Related references: PMID:15800615 PMID:20195506 PMID:21319273 PMID:28216226

• CDK2 phosphorylates the histone methyltransferase EZH2, promoting maintenance of H3K27me3 epigenetic marks and gene silencing. This highlights CDK2's role in epigenetic regulation. Related references: PMID:20935635

• Post-translational modifications of CDK2, such as phosphorylation, acetylation, and S-nitrosylation, modulate its activity and stability. CDK2 is a member of the protein kinase superfamily, featuring ATP-binding and metal-binding domains. Related references: PMID:20079829

Experimental Guidance and Technical Tips

• This antibody specifically recognizes CDK2 phosphorylated at Thr-160. To preserve phosphorylation during sample preparation, include phosphatase inhibitors in lysis buffers and avoid prolonged incubations at room temperature.
• For Western blotting, bands are expected at approximately 30 kDa and 34 kDa (corresponding to CDK2 isoforms or phosphorylation-induced shifts). Use λ‑phosphatase‑treated lysates as a negative control, and S‑phase‑enriched cell extracts as a positive control.
• In immunohistochemistry (IHC-P), antigen retrieval methods should be optimized to retain phospho‑epitopes. Validate staining specificity by comparing with total CDK2 staining or using peptide competition assays.
• For ELISA applications, the antibody can serve as a capture or detection reagent; titrate carefully and confirm linearity for quantitative phospho-CDK2-T160 detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Phospho-CDK2-T160 antibody is part of our extensive portfolio supporting cell cycle and oncology research, with applications in biomarker development and diagnostic assay design. For customized sourcing of antibody pairs, bulk ancillary reagents, or rare-target raw materials, our team is ready to support your projects with reliable supply and expert guidance.