Anti-Phospho-CDK1-Y15 Polyclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - P06493

Core Product Specifications and Parameters

Biological Background: CDK1 Function and Localization

• CDK1 (Cyclin-dependent kinase 1), also known as CDC2 or p34CDC2, is a serine/threonine kinase essential for eukaryotic cell cycle control, particularly the G2/M transition through association with interphase cyclins. Related references: PMID:16407259 PMID:16933150 PMID:17459720
• CDK1 phosphorylates lamin proteins (LMNA, LMNB1, LMNB2) at the onset of mitosis, triggering nuclear envelope breakdown. Related references: PMID:2188730 PMID:2344612 PMID:37788673
• Its activity is tightly regulated by phosphorylation: inhibitory phosphorylation at Tyr15 (and Thr14) by WEE1 and PKMYT1 kinases keeps CDK1 inactive; dephosphorylation by CDC25 phosphatases activates CDK1, driving mitotic entry. Related references: PMID:18480403 PMID:20360007 PMID:20395957
• CDK1 phosphorylates beta-tubulin to regulate microtubule dynamics during mitosis. Related references: PMID:16371510
• Subcellularly, CDK1 localizes to the nucleus, cytoplasm, mitochondria, centrosomes, and mitotic spindle, reflecting its diverse roles in cell division.
• Phosphorylation of Bcl-xL after prolonged G2 arrest induces apoptosis, while phosphorylation of CASP8 during mitosis prevents its proteolytic activation, demonstrating CDK1’s dual role in cell death regulation. Related references: PMID:19917720 PMID:20937773
• CDK1 also phosphorylates CGAS during mitosis to inhibit self-DNA sensing and regulates the circadian clock by phosphorylating NR1D1. Related references: PMID:32351706 PMID:27238018
• It is overexpressed in breast cancer tissues and acts as a receptor for hepatitis C virus entry into hepatocytes.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to Tyr15 of human CDK1; specificity for the phosphorylated form should be verified using phosphatase-treated lysates as a negative control.
• Expected molecular weight is approximately 34 kDa in western blotting; a band may be observed at this size under reducing conditions.
• Phosphorylation of CDK1 at Tyr15 is cell cycle-dependent; enrichment may be achieved by synchronizing cells or by treatment with phosphatase inhibitors.
• For immunofluorescence (IF-P), optimize fixation/permeabilization conditions as epitope accessibility may vary.
• Recommended sample species include human, mouse, and rat; cross-reactivity with other species should be independently validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and researchers. Our portfolio includes critical cell cycle targets like CDK1, enabling seamless procurement from concept to clinic. Contact us for rare-target antibody sourcing and technical support for your IVD development pipeline.