Anti-Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit Monoclonal Antibody for WB - P06493

Core Product Specifications and Parameters

Biological Background: CDK1 Function and Localization

• CDK1 is a cyclin-dependent kinase that governs G2-M transition by associating with multiple interphase cyclins, thereby controlling the eukaryotic cell cycle. Related references: PMID:16407259, PMID:16933150, PMID:17459720

• It phosphorylates a wide array of substrates including PARVA, APC, BRCA2, p53, and histones, coordinating centrosome cycle, mitotic onset, and chromosome condensation. Related references: PMID:16407259, PMID:16933150, PMID:17459720

• CDK1 is activated by the CDK‑activating kinase (CAK) complex (CDK7/cyclin‑H/MAT1) and inactivated through WEE1‑ and PKMYT1‑mediated phosphorylation, acting as a master mitotic switch. Related references: PMID:41100585, PMID:20360007

• Upon activation, CDK1 phosphorylates nuclear lamins (LMNA, LMNB1, LMNB2) to drive nuclear envelope breakdown at the onset of mitosis. Related references: PMID:2188730, PMID:2344612, PMID:37788673

• CDK1 localizes to the nucleus, cytoplasm, centrosome, and mitotic spindle, consistent with its pleiotropic roles in cell division.

• Phosphorylation of beta‑tubulins by CDK1 regulates microtubule dynamics during mitosis, a critical event for proper spindle formation. Related references: PMID:16371510

• During mitosis, CDK1 phosphorylates CGAS to prevent its activation by self DNA, thereby averting an autoinflammatory response. Related references: PMID:32351706

• Found in breast cancer tissues.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 34 kDa is expected; consider using mitotically synchronized cell lysates to enhance the signal.
• In immunofluorescence (IF‑P) and immunohistochemistry (IHC‑P), validate the phospho‑specific signal with appropriate controls, such as λ‑phosphatase treatment or blocking peptides.
• Due to cross‑reactivity with CDK2‑T160 and CDK3‑T160, additional bands corresponding to these kinases (~33‑35 kDa) may appear; confirm band identity with specific inhibitors or knockdown experiments.
• ELISA can be employed to quantify phospho‑CDK1 levels; include a non‑phosphorylated peptide control to assess specificity.
• For all applications, optimize antibody dilutions and fixation/permeabilization conditions in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, supporting projects from concept to clinic. This phospho‑specific CDK1 antibody serves as a valuable tool for studying cell cycle regulation, mitotic signaling, and their implications in cancer. Beyond primary antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing of rare‑target raw materials to accelerate your diagnostic and research workflows.