Anti-Phospho-CDK1-T14 Rabbit Polyclonal Antibody for WB, ELISA - P06493

Core Product Specifications and Parameters

Biological Background: CDK1 Function and Localization

• CDK1, also known as CDC2 or p34 protein kinase, is a serine/threonine-protein kinase that plays a key role in controlling the eukaryotic cell cycle by modulating the centrosome cycle and promoting the G2-M transition through association with multiple interphase cyclins.
  Related references: PMID:16407259, PMID:16933150, PMID:17459720
• CDK1 governs mitotic entry by phosphorylating a wide array of substrates involved in centrosome separation, Golgi dynamics, nuclear envelope breakdown, chromosome condensation, and spindle formation.
  Related references: PMID:18480403, PMID:20360007, PMID:2188730
• The activity of CDK1 is tightly regulated by phosphorylation; inhibitory phosphorylation at Thr14 (and Tyr15) by WEE1 and PKMYT1 kinases keeps CDK1 inactive during interphase, while dephosphorylation by CDC25 phosphatases triggers mitotic onset.
  Related references: PMID:18480403, PMID:20360007
• CDK1 localizes to multiple subcellular compartments, including the nucleus, cytoplasm, mitochondria, centrosome, and mitotic spindle, reflecting its diverse functions throughout the cell cycle.
• CDK1 acts as a host cell receptor for hepatitis C virus (HCV), facilitating viral entry into hepatocytes.
• CDK1 is a highly modified phosphoprotein, subject to acetylation, ubiquitination, and inhibitory phosphorylation at Thr14—a critical checkpoint controlling mitotic progression.
• Tissue specificity: CDK1 is notably found in breast cancer tissues, highlighting its importance in oncology research.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr14 of human CDK1, ensuring specific recognition of the phosphorylated epitope. For WB, consider verifying phospho-specificity by treating cell lysates with lambda phosphatase and comparing band intensity.
• This antibody has been validated for WB and ELISA. For WB, a recommended starting point is to test a range of lysate concentrations and antibody dilutions; a suitable loading control should be included.
• Cross-reactivity with human, mouse, and rat allows multi-species applications. When moving between species, confirm reactivity by testing a known positive control lysate.
• Given CDK1's role in mitosis, consider enriching for mitotic cells (e.g., by nocodazole synchronization) to enhance detection of the phosphorylated form.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers focused on cell cycle regulation and cancer biology, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This Anti-Phospho-CDK1-T14 antibody reflects our commitment to offering high-quality reagents for critical research applications.