Anti-Phospho-cdc25C (S216) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P30307

Core Product Specifications and Parameters

Biological Background: cdc25C Function and Localization

• The CDC25C gene encodes M-phase inducer phosphatase 3, a dual-specificity phosphatase that is a key regulator of the G2/M checkpoint.
• It functions as a dosage-dependent inducer of mitosis, dephosphorylating and activating CDK1 (cyclin-dependent kinase 1) to drive cell cycle progression.
  Related references: PMID:8119945
• Phosphorylation of cdc25C (e.g., at serine 216) modulates its activity and subcellular localization, with the phosphorylated form being highly effective in activating G2 cells into prophase.
• The protein is primarily localized to the nucleus, consistent with its role in controlling mitotic entry.
• cdc25C undergoes alternative splicing, producing isoforms with potentially distinct regulatory properties.
• Keywords associated with cdc25C include Acetylation, Phosphoprotein, Host-virus interaction, and Mitosis, highlighting its multifaceted regulation.

Experimental Guidance and Technical Tips

• Consider testing the antibody on phosphatase-treated samples to confirm phospho-specificity at S216.
• In Western blot, the phospho-cdc25C band may appear near 53 kDa; slight shifts due to phosphorylation can be expected.
• For IHC-P, evaluate citric acid or EDTA-based antigen retrieval techniques, mindful of possible phospho-epitope loss.
• When performing ELISA, using a phosphorylated peptide as the coating antigen can help confirm specificity.
• Given cross-reactivity with rodent species, this antibody may be applicable for mouse and rat model studies.
• Always include phosphatase inhibitors in lysis buffers to maintain S216 phosphorylation during sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including phospho-specific antibodies like this anti-cdc25C (S216) antibody, which is essential for studying cell cycle regulation and mitosis. From validated antibody pairs to bulk ancillary reagents and rare target sourcing, CamelBio streamlines your procurement needs from concept to clinic.