Anti-Phospho-CD117/c-Kit (Y721) Rabbit Polyclonal Antibody for WB - P10721

Core Product Specifications and Parameters

Biological Background: c-Kit Function and Localization

• KIT (CD117/c-Kit) is a receptor tyrosine kinase for stem cell factor (KITLG/SCF), playing critical roles in hematopoiesis, gametogenesis, and melanogenesis.
• Functions in cell survival, proliferation, stem cell maintenance, and mast cell development, migration, and function.
• Activates multiple signaling cascades including PI3K/AKT, RAS/RAF/MAPK, and JAK/STAT pathways.
• Phosphorylation at Tyr721 (corresponding to Tyr719 in mouse) is a key autophosphorylation event that facilitates binding and activation of downstream signaling molecules.
• Expressed in testis in spermatogonia, interstitial Leydig cells, and ejaculated spermatozoa. Related references: PMID:20601678
• Subcellularly localized predominantly at the cell membrane, with some cytoplasmic presence.
• Belongs to the immunoglobulin superfamily and contains a kinase domain; post-translational modifications include glycosylation and ubiquitination.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide corresponding to residues surrounding Tyr719 of mouse Kit. Cross-reactivity with human and rat is expected due to high sequence homology.
• For Western blot, a band around 110–145 kDa (mature glycosylated form) may be detected; consider using appropriate stimulation conditions to induce tyrosine phosphorylation.
• ELISA may be used for detecting phospho-c-Kit in cell lysates; validate with positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to validated IVD raw materials, including premium phospho-specific antibodies like this anti-Phospho-CD117/c-Kit-Y721 rabbit polyclonal antibody. With a comprehensive portfolio spanning validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, CamelBio supports your assay development from target discovery to clinical validation, particularly for oncology and stem cell research applications.