Anti-Phospho-CD117/c-Kit-Y721 Rabbit pAb for WB - P10721

Core Product Specifications and Parameters

Biological Background: Mast/stem cell growth factor receptor Kit Function and Localization

• KIT, also known as proto-oncogene c-Kit, CD117, or mast/stem cell growth factor receptor, is a receptor tyrosine kinase encoded by the KIT gene.
• It functions as the cell-surface receptor for the cytokine stem cell factor (SCF/KITLG) and is essential for regulating cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis.
• Upon ligand binding, KIT activates multiple downstream signaling pathways, including PI3K/AKT, RAS/RAF/MAPK, and JAK/STAT cascades, resulting in the phosphorylation of various substrates such as PIK3R1, PLCG1, CBL, and members of the STAT family.
• The receptor is primarily localized to the cell membrane and can also be found in the cytoplasm.
• At the protein level, KIT is detected in spermatogonia and interstitial Leydig cells in the testis, and its expression is maintained in ejaculated spermatozoa.
  Related references: PMID:20601678
• KIT is subject to post-translational modifications, including ligand-induced phosphorylation and ubiquitination, which regulate its activity and internalization.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic phospho-specific peptide corresponding to residues surrounding tyrosine 721 (Y721) of human KIT; consider using SCF-stimulated cell lysates as a positive control to verify phosphorylation-dependent detection.
• For Western blot, validate the antibody in the relevant sample system; use non-phosphorylated peptide blocking or phosphatase-treated samples to confirm specificity for the phosphorylated form.
• The predicted molecular weight of KIT is approximately 110 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• Cross-reactivity is confirmed for human; for other species, check the sequence homology around the Y721 phosphorylation site before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-phospho-CD117/c-Kit antibody supports signal transduction and oncology research, and our team can assist with sourcing rare or custom targets to streamline your assay development from concept to clinic.