Anti-Phospho-Caveolin-1-Y14 Rabbit Polyclonal Antibody for WB and ELISA - Q03135

Core Product Specifications and Parameters

Biological Background: Caveolin-1 Function and Localization

• Caveolin-1 (CAV1) is the principal scaffolding protein of caveolar membranes, forming stable hetero-oligomeric complexes with CAV2 to drive caveolae formation and recruit CAVIN proteins.
  Related references:
  PMID:11751885
  PMID:19262564
• It directly binds G protein alpha subunits, functionally regulating their activity, and participates in T-cell receptor-mediated activation by interacting with DPP4 to induce T-cell proliferation and NF-kappa-B activation.
  Related references:
  PMID:17287217
• CAV1 regulates multiple signaling pathways: it recruits CTNNB1 to caveolar membranes (Wnt pathway) and negatively modulates TGFB1 signaling by promoting internalization and degradation of TGFBR1.
  Related references:
  PMID:25893292
• The protein localizes to the Golgi apparatus membrane, cell membrane, caveolae, membrane rafts, trans-Golgi network, and cytoplasm.
• Post-translational modifications include phosphorylation, acetylation, palmitoylation, and ubiquitination, with Y14 phosphorylation being a key regulatory event.
• Caveolin-1 is expressed in various tissues, including skeletal muscle, liver, stomach, lung, kidney, heart, and brain.
• Mutations in CAV1 are associated with congenital generalized lipodystrophy and diabetes mellitus, and the protein plays a role in host–virus interactions.

Experimental Guidance and Technical Tips

• This antibody is raised against a phosphorylated peptide around Y14; it specifically recognizes the phosphorylated form of Caveolin-1. For Western blot, ensure that samples are prepared with phosphatase inhibitors and use appropriate blocking conditions to preserve phospho-epitopes.
• A band at approximately 20 kDa is expected for Caveolin-1 in human and mouse lysates. Consider loading a dephosphorylated control to confirm phospho-specificity.
• The antibody has been applied in ELISA, which may be useful for screening or quantifying phospho-Caveolin-1 levels. Verify optimal working dilutions experimentally for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier, offering a comprehensive portfolio including this Phospho-Caveolin-1-Y14 antibody and other signaling research tools. As a one-stop source, we support diagnostic manufacturers and laboratories with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and the ability to source rare targets—all backed by technical consultation to streamline your development from concept to clinic. Contact us to discuss your caveolae signaling and biomarker project needs.