Anti-Phospho-Camk2-T286 Rabbit Monoclonal Antibody for WB - Q13554 / Q13557 / Q13555 / Q9UQM7

Core Product Specifications and Parameters

Biological Background: Camk2 (CaMKII) Function and Localization

• CaMKII is a serine/threonine-protein kinase that functions as a holoenzyme complex, with the beta subunit (CAMK2B) playing a key role in neuronal development and plasticity (PubMed:16690701, PubMed:29100089). Related references: PMID:16690701, PMID:29100089
• In neurons, CaMKII beta exhibits a kinase-independent structural function by binding and bundling actin filaments, essential for dendritic spine morphogenesis and synaptic plasticity (PubMed:29100089). Related references: PMID:29100089
• Autophosphorylation of CaMKII (including at Thr286 in many isoforms) upon Ca2+/calmodulin binding generates Ca2+-independent autonomous kinase activity, critical for long-term potentiation (LTP) and hippocampus-dependent learning (PubMed:16690701, PubMed:29100089). Related references: PMID:16690701, PMID:29100089
• In skeletal muscle, CaMKII beta regulates sarcoplasmic reticulum Ca2+ transport by phosphorylating triadin and phospholamban, modulating muscle function in response to exercise (PubMed:16690701). Related references: PMID:16690701
• Under ER stress, CaMKII beta phosphorylates reticulophagy regulator RETREG1 at Ser-151, promoting its oligomerization and membrane scission activity for reticulophagy (PubMed:31930741). Related references: PMID:31930741
• The protein localizes to multiple cellular compartments, including the cytoplasm, cytoskeleton, centrosome, sarcoplasmic reticulum membrane, and synapses, reflecting its diverse roles.
• CaMKII beta is widely expressed, with high levels in adult and fetal brain and skeletal muscle, consistent with its functions in neurogenesis and muscle physiology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide around T286 of human Camk2; this site is conserved across CAMK2A/B/G/D isoforms and in mouse, supporting the observed cross-reactivity. Consider validating the antibody in mouse brain or muscle lysates.
• For western blotting, expect a band at approximately 54 kDa corresponding to phosphorylated Camk2. Include appropriate loading controls and, if possible, a phosphatase-treated sample to confirm phosphorylation specificity.
• In ELISA, use the phospho-peptide as the coating antigen and the corresponding non-phosphorylated peptide as a negative control to assess specificity.
• Since the antibody recognizes an activation-dependent phosphorylation, optimize stimulation conditions (e.g., Ca2+ ionophore or neurotransmitter treatment) for robust detection in cellular models.
• Determine the optimal working dilution for each application experimentally; recommended starting dilutions are typically 1:500–1:2000 for WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies for neuroscience and cell signaling research. This Phospho-Camk2-T286 Rabbit mAb exemplifies our commitment to offering high-quality reagents for detecting critical post-translational modifications in pathways such as synaptic plasticity and muscle function. Beyond catalog products, CamelBio offers one-stop access to ancillary reagents, custom antibody development, and rare target sourcing to streamline your assay development and scale-up needs.