Anti-Phospho-Camk2-T286 Polyclonal Antibody for WB, ELISA - Q13554 / Q13557 / Q13555 / Q9UQM7

Core Product Specifications and Parameters

Biological Background: CaMKII Function and Localization

• CaMKII (Calcium/calmodulin-dependent protein kinase II) is a serine/threonine kinase forming a holoenzyme of α, β, γ, and δ subunits. The antibody detects phosphorylated T286 on the α subunit (CAMK2A), a key autophosphorylation site that locks the kinase in a calcium-independent active state.
• In neurons, CaMKII is critical for dendritic spine maturation, synapse formation, and long-term potentiation (LTP), acting downstream of NMDA receptors to sustain synaptic plasticity and hippocampus-dependent learning. Related references: PMID:29100089
• The β subunit (CAMK2B) also has a structural role, binding and bundling actin filaments in a kinase-independent manner to target CAMK2A to synapses and remodel the actin cytoskeleton during plasticity.
• In skeletal muscle, CaMKII contributes to exercise-induced adaptation by regulating sarcoplasmic reticulum Ca²⁺ handling: in slow‑twitch fibers it modulates Ca²⁺ transport, and in fast‑twitch fibers it controls Ca²⁺ release via phosphorylation of triadin and phospholamban. Related references: PMID:16690701
• Under endoplasmic reticulum stress, CaMKII phosphorylates the reticulophagy regulator RETREG1 at Ser‑151, promoting its oligomerization and membrane scission to enhance ER‑phagy. Related references: PMID:31930741
• The kinase localizes to the cytoplasm, cytoskeleton, centrosome, sarcoplasmic reticulum membrane, and synapse, consistent with its diverse neuronal and muscular roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide spanning T286 of human Camk2a; thus, the antibody selectively recognizes the phosphorylated form of this conserved site. For accurate phosphorylation analysis, include phosphatase inhibitors in lysis and assay buffers.
• For Western blotting, validate band specificity by treating samples with λ‑phosphatase or competition with the non‑phosphopeptide. The predicted molecular weight of α‑CaMKII is ~54 kDa, but other isoforms may appear due to cross‑reactivity.
• In ELISA applications, confirm phospho‑specific binding by comparing signals against phosphorylated versus unphosphorylated peptide standards.
• The reported cross‑reactivity with mouse and rat indicates high sequence conservation of the T286 epitope; however, always verify detection in the relevant species and tissue type before proceeding with quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes with a comprehensive, one‑stop supply of IVD raw materials – from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing. This Phospho‑Camk2‑T286 antibody aligns with our portfolio for neuroscience and signal‑transduction research, supporting your assay development with batch‑to‑batch consistency and expert technical guidance. Contact us to discuss how we can help streamline your path from concept to clinic.