Anti-Phospho-c-Myc (T58) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01106

Core Product Specifications and Parameters

Biological Background: Myc proto-oncogene protein Function and Localization

• Myc proto-oncogene protein, also known as c-Myc, is a member of the basic helix-loop-helix leucine zipper (bHLHZip) family of transcription factors. It is encoded by the MYC gene (Gene ID: 4609) and plays a central role in cell cycle progression, apoptosis, and cellular transformation.
• c-Myc binds DNA in a sequence-specific manner, recognizing the E-box consensus sequence 5'-CAC[GA]TG-3', and activates the transcription of numerous growth-related genes. Related references: PMID:24940000 PMID:25956029
• It directly binds to the VEGFA promoter and promotes VEGFA production, thereby stimulating sprouting angiogenesis. Related references: PMID:24940000 PMID:25956029
• c-Myc functions as a regulator of somatic cell reprogramming and controls the self-renewal capacity of embryonic stem cells.
• Together with the transcription initiation factor TAF6L, c-Myc activates target gene expression by promoting the release of paused RNA polymerase II.
• c-Myc positively regulates the transcription of HNRNPA1, HNRNPA2, and PTBP1, which in turn modulate the alternative splicing of pyruvate kinase PKM, favoring the production of the PKM2 isoform. Related references: PMID:20010808
• Subcellular localization includes the nucleus (nucleoplasm, nucleolus), cytoplasm, and chromosomes, reflecting its dynamic roles in transcription and genome organization.
• c-Myc undergoes extensive post-translational modifications, including phosphorylation (notably at T58), acetylation, ubiquitination, and O-linked glycosylation, which tightly regulate its stability, protein interactions, and transcriptional activity.

Experimental Guidance and Technical Tips

• Since this antibody specifically recognizes the phosphorylated T58 residue, include phosphatase inhibitors in your lysis buffer and during sample preparation to preserve the phospho-signal.
• For Western blotting, the predicted molecular weight is 51 kDa, but you may observe bands at higher apparent molecular weights (57–65 kDa) due to post-translational modifications; always run a molecular weight marker.
• Confirm antibody specificity by treating samples with lambda protein phosphatase or by peptide competition with the non-phosphorylated immunogen if available.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and primary antibody dilution; a suggested starting dilution for immunohistochemistry is 1:50–1:200, but this must be validated in your own system.
• This antibody has been reported to react with human, mouse, and rat samples; when working with other species, test cross-reactivity empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a single point of access to a wide range of IVD raw materials, supporting every stage from concept to clinical application. Our phospho-c-Myc (T58) antibody is part of a growing portfolio of oncology-related reagents, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. For custom sourcing, scale-up, or technical consultation on rare or custom targets, contact CamelBio to accelerate your project while maintaining quality and traceability.