Anti-Phospho-c-Myc (T358) Polyclonal Antibody for WB, ELISA - P01106

Core Product Specifications and Parameters

Biological Background: c-Myc Function and Localization

• The c-Myc proto-oncogene protein (also known as MRTL, MYCC, bHLHe39) is a transcription factor that plays central roles in cell proliferation, growth, and apoptosis (PubMed:24940000, PubMed:25956029). Related references: PMID:24940000, PMID:25956029
• It binds both non-specifically and specifically to E-box motifs (5'-CAC[GA]TG-3') in promoters, activating growth-related genes and VEGFA to promote angiogenesis (PubMed:24940000, PubMed:25956029).
• Regulates somatic reprogramming and maintains self-renewal in embryonic stem cells (By similarity).
• Functions with TAF6L to release paused RNA polymerase II and positively regulates splicing factors HNRNPA1, HNRNPA2, and PTBP1, thereby modulating PKM alternative splicing to favor the M2 isoform (PubMed:20010808). Related references: PMID:20010808
• Localizes predominantly to the nucleus (nucleoplasm and nucleolus), but also appears in the cytoplasm and associates with chromosomes.
• Post-translationally modified by acetylation, ubiquitination, and phosphorylation. Phosphorylation at Thr358 (the epitope of this antibody) is one of many regulatory events influencing c-Myc stability and activity.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is 51 kDa; however, due to extensive post-translational modifications, bands may appear at different sizes. Validate the antibody with appropriate positive and negative controls in your cell system.
• In ELISA, use the phospho-specific peptide for coating or competition to confirm specificity for the T358 phosphorylated form. Non-phosphorylated peptide may be employed as a negative control.
• Optimize primary antibody concentration for each application; starting points for WB are often between 1:500 and 1:2000, but empirical determination is recommended.
• Keep the antibody on ice during handling and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this high-quality anti-phospho-c-Myc (T358) polyclonal antibody as part of our extensive IVD raw-material portfolio. We empower diagnostic manufacturers and researchers with reliable reagents for oncogene and signal transduction studies, including validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. Partner with us for tailored sourcing solutions that accelerate your assay development from concept to clinic.