Anti-Phospho-c-Myc (S62) Rabbit Polyclonal Antibody for WB, IHC-P, and ELISA - P01106

Core Product Specifications and Parameters

Biological Background: Myc proto-oncogene protein Function and Localization

• MYC (c-Myc) is a well-known proto-oncogene and transcription factor, also designated as class E basic helix-loop-helix protein 39 (bHLHe39) or transcription factor p64. It is encoded by the MYC gene and is closely associated with cell growth, proliferation, and oncogenesis.
• As a sequence-specific transcription factor, MYC binds the canonical E-box motif 5'-CACGTG-3' to activate the transcription of numerous growth-related genes, thereby driving cell cycle progression and cellular proliferation.
  Related references:
  PMID:24940000 PMID:25956029
• MYC upregulates VEGFA expression by directly binding to its promoter, thereby promoting sprouting angiogenesis in both physiological and pathological contexts.
  Related references:
  PMID:24940000 PMID:25956029
• In embryonic stem cells, MYC functions as a key regulator of somatic reprogramming and self-renewal, cooperating with TAF6L to facilitate RNA polymerase II pause release and target gene activation (by similarity).
• MYC positively regulates the expression of HNRNPA1, HNRNPA2, and PTBP1, which collectively modulate alternative splicing of the pyruvate kinase PKM pre-mRNA, favoring the production of the PKM M2 isoform. This mechanism is crucial for the metabolic reprogramming observed in many cancers.
  Related references:
  PMID:20010808
• Subcellularly, MYC predominantly localizes to the nucleus—specifically the nucleoplasm and nucleolus—but can also shuttle into the cytoplasm and associate with chromosomes, reflecting its dynamic role in transcriptional regulation and chromatin dynamics.
• The protein is subject to extensive post-translational modifications, including phosphorylation at multiple serine and threonine residues (such as S62), acetylation, O-glycosylation, and ubiquitination. These modifications tightly regulate MYC protein stability, activity, and interactome.

Experimental Guidance and Technical Tips

• This antibody is raised against a phospho‑specific immunogen (human c‑Myc peptide phosphorylated at Ser62). For western blot (WB), it is recommended to prepare cell or tissue lysates with phosphatase inhibitors and validate phospho‑specificity using λ‑phosphatase treatment or competing non‑phosphopeptide.
• For immunohistochemistry (IHC‑P), standard antigen retrieval and blocking steps are advised, and the antibody may be tested at several dilutions to optimize signal‑to‑background ratio.
• In ELISA, the antibody can be used as a capture or detection reagent; however, individual assay optimization is required, and cross‑reactivity with unphosphorylated c‑Myc should be assessed.
• Given the predicted protein weight of approximately 51 kDa, use an appropriate molecular‑weight marker and confirm the correct band in the relevant sample system (e.g., human, mouse, or rat cell lines).
• Consider using this antibody in combination with total c‑Myc antibodies to evaluate phosphorylation status in signaling studies (e.g., MAPK/ERK pathway).

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