Anti-Phospho-c-Myc-S373 Rabbit Polyclonal Antibody for WB - P01106

Core Product Specifications and Parameters

Biological Background: c-Myc Function and Localization

• c-Myc (MYC proto-oncogene protein), also known as bHLHe39 or transcription factor p64, is a member of the basic helix-loop-helix (bHLH) transcription factor family.
• It functions as a transcription factor that specifically recognizes E-box sequences (5'-CAC[GA]TG-3') and activates the expression of genes involved in cell growth, proliferation, and angiogenesis via VEGFA promoter binding. Related references: PMID:24940000 PMID:25956029
• c-Myc also regulates alternative splicing: it activates transcription of HNRNPA1, HNRNPA2, and PTBP1, which repress exon 9 inclusion in PKM pre-mRNA, favoring the PKM2 isoform production. Related references: PMID:20010808
• Subcellularly, c-Myc is predominantly found in the nucleus (nucleoplasm and nucleolus) but can also localize to the cytoplasm and associate with chromosomes.
• c-Myc is subject to multiple post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which regulate its stability, activity, and degradation.

Experimental Guidance and Technical Tips

• This antibody was generated against a phospho-specific peptide surrounding Ser373 of human c-Myc. Verify phospho-specificity using phosphatase-treated samples or non-phosphopeptide competition in your experimental system.
• For Western blot analysis, optimal working dilutions should be determined empirically. Consider starting with a standard range (e.g., 1:500–1:2000) and adjust based on signal-to-noise ratio.
• To preserve phosphorylation signals, use lysis buffers supplemented with phosphatase inhibitors and avoid prolonged sample processing at room temperature.
• A band corresponding to the predicted molecular weight (~51 kDa) may be detected, but post-translational modifications or splice variants could give rise to additional bands.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and rare-target sourcing to accelerate diagnostic development. This anti-phospho-c-Myc-S373 antibody extends our portfolio for oncology and transcription research, and we can assist in scaling your procurement from initial R&D to full commercial production. Reach out to explore how we can streamline your c-Myc-related assay development with one-stop service and supply.