Anti-Phospho-c-Jun (T91) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P05412

Core Product Specifications and Parameters

Biological Background: c-Jun Function and Localization

• c-Jun is a transcription factor encoded by the JUN gene, also known as AP-1, p39, and proto-oncogene c-Jun. It is a critical component of the AP-1 transcription complex.
• It recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). c-Jun heterodimerizes with FOS family proteins, enhancing DNA binding and transcriptional activity. Related references: PMID:10995748 | PMID:22083952
• Together with FOSB, c-Jun induces transcription of FASLG/CD95L in T cells, thereby promoting activation-induced cell death (PubMed:12618758). Related references: PMID:12618758
• When phosphorylated by HIPK3, c-Jun promotes NR5A1 activity, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Related references: PMID:17210646
• In colorectal cancer (CRC) cells, c-Jun is involved in activated KRAS-mediated transcriptional activation of USP28 by binding to the USP28 promoter (PubMed:24623306). Related references: PMID:24623306
• c-Jun is primarily localized to the nucleus, consistent with its function as a transcription factor.
• It is expressed in the developing and adult prostate, as well as in prostate cancer cells.
• Post-translational modifications of c-Jun include phosphorylation, acetylation, and ubiquitination. The protein is a known phosphoprotein, with threonine 91 being a regulatory phosphorylation site.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide around T91 of human c-Jun. Confirm phosphorylation specificity by comparing signals from phosphorylated and non-phosphorylated peptide competition assays or by treating lysates with phosphatase.
• For Western blotting, a band of approximately 36 kDa is expected under standard denaturing and reducing conditions. Include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and validate using positive and negative control tissues known to express phosphorylated c-Jun.
• For ELISA, this antibody may serve as a detection reagent; validation in your specific assay format with appropriate controls is recommended.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide comprehensive one-stop access to IVD raw materials, including validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents, supporting diagnostic kit development from concept to clinic. Our catalog includes high-quality phosphorylation-specific antibodies like this anti-phospho-c-Jun (T91) monoclonal antibody, aiding research into AP-1 signaling, oncogenesis, and immune responses. For custom sourcing of rare-target raw materials, CamelBio is your trusted partner to accelerate assay development.