Anti-Phospho-c-Jun-S63 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P05412

Core Product Specifications and Parameters

Biological Background: c-Jun Function and Localization

• Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3', regulating gene expression.
  Related references:
  PMID:10995748
  PMID:22083952
• Heterodimerizes with FOS family proteins to form AP-1 complexes, enhancing DNA binding and transcriptional activity (By similarity).
• Plays a role in activation-induced cell death of T cells by inducing FASLG/CD95L expression upon TCR/CD3 signaling.
  Related references:
  PMID:12618758
• Promotes NR5A1 activity when phosphorylated, leading to increased steroidogenic gene expression via cAMP signaling.
  Related references:
  PMID:17210646
• Involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells, binding to the USP28 promoter.
  Related references:
  PMID:24623306
• Upon EBV infection, c-Jun binds to the viral BZLF1 Z promoter and activates BZLF1 expression, facilitating the viral lytic cycle.
• Localizes to the nucleus and is expressed in developing and adult prostate tissues, as well as prostate cancer cells.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which modulate its stability, activity, and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser63; adding phosphatase inhibitors during sample preparation is recommended to preserve the phospho-signal.
• For Western blotting, a band at ~36 kDa is expected; validate with appropriate positive and negative controls (e.g., phosphatase-treated samples).
• In immunofluorescence/ICC, optimization of fixation and permeabilization conditions may be necessary to maintain epitope recognition of the phosphorylated target.
• Cross-reactivity with human, mouse, and rat c-Jun has been confirmed; for other species, align target sequences around the phosphorylation site to assess potential reactivity.
• For ELISA applications, consider using peptide-based assays to quantify phosphorylation levels; pair with non-phospho-specific antibodies for ratio analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined, one-stop source for IVD antibodies, recombinant proteins, and ancillary reagents. For transcription factor and oncology research, our portfolio includes validated phospho-specific monoclonal antibodies like this anti-c-Jun (S63) clone, along with antibody pairs, bulk conjugates, and customized raw-material sourcing. From early assay development to clinical validation, our technical consulting and supply chain capabilities support your end-to-end workflow needs.