Anti-Phospho-c-Abl-Y245 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P00519

Core Product Specifications and Parameters

Biological Background: ABL1 Function and Localization

• ABL1 is a non-receptor tyrosine kinase involved in diverse processes including actin remodeling, cell adhesion, receptor endocytosis, autophagy, DNA damage response, and apoptosis.
• Phosphorylation of WASF3 by ABL1 is critical for lamellipodia formation and cell migration. Related references: PMID:22810897
• In the nucleus, ABL1 participates in DNA repair and apoptotic responses, phosphorylating substrates such as TP73 and CASP9.
• The protein shuttles between the cytoplasm, cytoskeleton, nucleus, and mitochondria, with nuclear translocation linked to DNA damage and mitochondrial targeting linked to oxidative stress.
• In response to oxidative stress, ABL1 phosphorylates PRKD2 at Tyr-717, mediating mitochondrial dysfunction and cell death. Related references: PMID:28428613
• ABL1 is subject to extensive post-translational regulation, including phosphorylation (e.g., at Y245), acetylation, N-myristoylation, and ubiquitination.
• Chromosomal translocations fusing ABL1 with BCR produce the BCR-ABL1 oncoprotein, a hallmark of chronic myeloid leukemia (CML).
• ABL1 is widely expressed across tissues.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a synthetic phosphopeptide corresponding to residues surrounding Tyr245 of human ABL1. It is recommended to treat cell or tissue lysates with phosphatase inhibitors to preserve phosphorylation.
• For Western blotting, the predicted molecular weight is approximately 123 kDa; validate the band in stimulated vs. unstimulated samples or treat with phosphatases as a specificity control.
• In IHC-P, antigen retrieval and phosphatase inhibitor pretreatment of tissue sections may be required to retain the phospho-epitope.
• Consider using ELISA with phosphopeptide and non-phosphopeptide controls to confirm phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated IVD raw-material partner, supplying validated monoclonal and polyclonal antibodies, including this anti-Phospho-c-Abl-Y245 antibody, for kinase signaling research and oncology applications. We offer one-stop access to bulk ancillary reagents, rare target sourcing, and technical consultation to streamline your diagnostic development from concept to clinic.