Anti-Phospho-BTK (Tyr223) Rabbit Monoclonal Antibody for WB and ELISA - Q06187

Core Product Specifications and Parameters

Biological Background: BTK Function and Localization

• Tyrosine-protein kinase BTK (Bruton tyrosine kinase) is a non-receptor tyrosine kinase essential for B-cell development, differentiation, and antigen receptor (BCR) signaling. It phosphorylates PLCG2 in cooperation with BLNK, triggering calcium mobilization and downstream PKC activation. Related references: PMID:19290921 PMID:11606584
• BTK acts as a signaling platform in cytokine receptor pathways and is a key component of the Toll-like receptor (TLR) pathway. It critically regulates TLR9 activation in splenic B-cells and induces TIRAP phosphorylation and degradation. Related references: PMID:16517732 PMID:17932028 PMID:16415872
• BTK regulates transcription by activating NF-kappa-B, linking TLR8/9 to NF-kappa-B, and directly phosphorylating transcription factors such as GTF2I and ARID3A to modulate gene expression. Related references: PMID:19290921 PMID:9012831 PMID:16738337
• BTK has a dual role in apoptosis and activates the NLRP3 inflammasome by phosphorylating NLRP3. Related references: PMID:9751072 PMID:34554188
• BTK contributes to STING1-mediated type I interferon responses by phosphorylating DDX41. Related references: PMID:25704810
• Subcellularly, BTK localizes to the cytoplasm, cell membrane, nucleus, and membrane rafts.
• Predominantly expressed in B-lymphocytes.
• Associated with keywords such as acetylation, alternative promoter usage, disease variant (X-linked agammaglobulinemia), SH2/SH3 domains, and zinc-finger, reflecting its diverse regulatory roles.

Experimental Guidance and Technical Tips

• Designed against a phosphorylated peptide around Tyr223, this antibody is phosphorylation-site specific. Validate phospho‑specificity by treating samples with phosphatase or using non‑phospho‑specific controls.
• For Western blotting, consider including phosphatase inhibitors during lysis and using BSA‑based blocking buffers to reduce non‑specific binding of phospho‑antibodies.
• Validate antibody performance in your target sample system, as cross‑reactivity is reported for human samples. Titrate the antibody for optimal signal in your ELISA setup.
• As a rabbit monoclonal antibody, expect consistent lot‑to‑lot reproducibility and high affinity, suitable for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this phospho‑specific BTK antibody, to support diagnostic and research laboratories. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing capabilities. Partner with CamelBio to accelerate your B‑cell signaling and immune function assay development with reliable supply solutions.