Anti-Phospho-BRD4 (Ser1070) Rabbit Polyclonal Antibody for WB, ELISA - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• BRD4 (Bromodomain-containing protein 4), also known as HUNK1, is a chromatin reader that binds acetylated histones and transmits epigenetic memory across cell divisions. It remains associated with chromatin throughout the cell cycle, helping maintain chromatin structure and enabling postmitotic transcription (PubMed:20871596, PubMed:23086925, PubMed:23317504, PubMed:22334664, PubMed:23589332). Related references: PMID:20871596 PMID:23086925 PMID:23317504 PMID:22334664 PMID:23589332

• It recruits the P-TEFb complex to promoters and enhancers, promoting phosphorylation of the RNA polymerase II C-terminal domain (CTD) at Ser2 and facilitating transcriptional elongation. JMJD6 collaborates with BRD4 to displace negative regulators such as HEXIM1 and 7SK snRNA (PubMed:16109376, PubMed:16109377, PubMed:19596240). Related references: PMID:16109376 PMID:16109377 PMID:19596240

• BRD4 recognizes acetylated RELA, leading to recruitment of P-TEFb and activation of NF-kappa-B signaling (PubMed:19103749). Related references: PMID:19103749

• Following phosphorylation by CK2, BRD4 is recruited to p53/TP53 target promoters and regulates p53-mediated transcription (PubMed:23317504). Related references: PMID:23317504

• In the DNA damage response, BRD4 functions as a chromatin insulator by recruiting the condensin-2 complex to acetylated histones, limiting the spread of H2AX phosphorylation and modulating signaling.

• BRD4 is predominantly localized to the nucleus and chromosomes, consistent with its role in chromatin regulation. It is ubiquitously expressed and undergoes various post-translational modifications, including phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• To maintain BRD4 phosphorylation at Ser1070 during sample preparation, add phosphatase inhibitors to lysis buffers and avoid prolonged incubation at room temperature.
• For western blotting (WB), the predicted molecular weight of BRD4 is 152 kDa; confirm band identity using appropriate positive and negative controls (e.g., phosphatase-treated lysates).
• ELISA experiments should include phosphorylated and non-phosphorylated peptide controls to verify antibody specificity for the phospho-epitope.
• Optimize antibody dilution and incubation conditions empirically; typical starting dilutions for WB range from 1:500 to 1:2000.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing one-stop access to validated antibodies, bulk ancillary reagents, and custom sourcing services for chromatin and transcription research. Whether you need phospho-specific antibodies like this anti-BRD4 (pS1070) product, matched antibody pairs, or rare target reagents, our team supports diagnostic developers and labs from concept to clinical application.