Anti-Phospho-BCR (Y177) Rabbit Polyclonal Antibody for WB, ELISA - P11274

Core Product Specifications and Parameters

Biological Background: BCR Function and Localization

• Encoded by the BCR gene (GeneID 613), the breakpoint cluster region protein (also known as renal carcinoma antigen NY-REN-26) is a key signaling molecule.
• BCR has dual regulatory roles: its C-terminal GAP domain accelerates GTP hydrolysis by RAC1, RAC2, and CDC42, while its central DH domain serves as a GEF that activates CDC42, RHOA, and RAC1.
  Related references: PMID:17116687, PMID:1903516, PMID:7479768, PMID:23940119
• The N-terminal region possesses intrinsic serine/threonine kinase activity.
  Related references: PMID:1657398
• BCR acts as a negative regulator of neuronal RAC1 and localizes to postsynaptic densities, dendritic spines, axons, and synapses.
• In macrophages, BCR modulates CSF1-directed motility and phagocytosis via RAC1.
  Related references: PMID:17116687
• In keratinocytes, BCR functions as a RHOA GEF, contributing to focal adhesion formation and differentiation.
  Related references: PMID:23940119
• As a proto-oncogene, BCR is involved in chromosomal rearrangements, most notably the t(9;22) translocation producing BCR-ABL fusions in chronic myeloid leukemia and acute lymphoblastic leukemia.
• This antibody specifically recognizes BCR phosphorylated at tyrosine 177 (Y177).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Y177; for WB, consider using phosphatase inhibitors during sample preparation to preserve phosphorylation.
• Expected band size ~143 kDa; validate detection in positive control lysates known to express phosphorylated BCR (e.g., BCR-ABL-positive cell lines).
• For ELISA, the antibody may be used in a phospho-specific sandwich format; pairing with a total BCR antibody could allow normalization.
• Cross-reactivity with mouse enables use in murine models; verify expression patterns in relevant tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing platform for IVD raw materials, including this anti-Phospho-BCR (Y177) antibody tailored for kinase signaling and oncology research. Our comprehensive portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting your diagnostic development from concept to clinic.