Anti-Phospho-Bcl-XL (S62) Rabbit Polyclonal Antibody for WB, IF/ICC - Q07817

Core Product Specifications and Parameters

Biological Background: Bcl-XL (Bcl-2-like protein 1) Function and Localization

• Bcl-XL is a member of the Bcl-2 family and potently inhibits apoptosis by binding to VDAC, preventing CYC1 release, and blocking caspase activation.
• Isoform Bcl-X(L) regulates presynaptic plasticity, enhancing mitochondrial ATP production and facilitating synaptic vesicle endocytosis via GTPase DNM1L.
• Bcl-X(L) may exert anti-inflammatory effects by inhibiting NLRP1-inflammasome activation and subsequent IL1B release. Related references: PMID:17418785
• The short isoform Bcl-X(S) promotes apoptosis, opposing the function of Bcl-X(L).
• Subcellularly, Bcl-X(L) resides in mitochondrial membranes, cytosol, nuclei, and synaptic vesicles, while also associating with centrosomes.
• Tissue-specific expression: Bcl-X(L) is prevalent in long-lived postmitotic cells (e.g., adult brain), whereas Bcl-X(S) dominates in rapidly turning-over cells like developing lymphocytes.
• The S62 phosphorylation site (target of this antibody) is a post-translational modification that may regulate Bcl-XL function and stability.

Experimental Guidance and Technical Tips

• This antibody targets a phosphorylation-specific epitope; include phosphatase treatment or phospho-deficient mutant controls to confirm specificity.
• For Western blot, an expected band at ~26 kDa should be observed; verify with known Bcl-XL-expressing cell lines (e.g., HeLa, Jurkat).
• In immunofluorescence, optimize fixation and permeabilization to preserve phospho-epitopes; co-localization with mitochondrial markers can validate localization.
• Although cross-reactivity with mouse and rat is reported, individual optimization is recommended for rodent sample analysis.
• Consider that post-translational modifications beyond phosphorylation may cause molecular weight shifts; always refer to positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a unified sourcing platform for IVD raw materials, technical services, and consulting. For apoptosis and Bcl-XL pathway investigations, we supply validated antibody reagents, antigen pairs, and ancillary materials tailored to your assay development needs. Our global network enables reliable access to specialized raw materials, helping you streamline from concept to clinical application.