Anti-Phospho-Bcl-2-S70 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P10415

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Bcl-2 is a well-characterized anti-apoptotic protein that suppresses cell death in various cell types, including factor-dependent lymphohematopoietic and neural cells. Related references: PMID:1508712 PMID:8183370
• It regulates apoptosis by controlling mitochondrial membrane permeability and feedback loops with caspases, inhibiting cytochrome c release and binding to APAF-1. Related references: PMID:11368354
• Bcl-2 also functions as an inhibitor of autophagy by interacting with BECN1 and AMBRA1 under non-starvation conditions. Related references: PMID:18570871 PMID:20889974 PMID:21358617
• Additionally, Bcl-2 may attenuate inflammation by impairing NLRP1-inflammasome activation, reducing CASP1 activation and IL1B release. Related references: PMID:17418785
• The protein is localized to multiple membranes, including the mitochondrial outer membrane, nuclear membrane, and endoplasmic reticulum membrane, as well as the cytoplasm.
• Bcl-2 is subject to post-translational modifications, including phosphorylation at serine 70, which can regulate its activity and stability. It is also identified as a phosphoprotein and a proto-oncogene.
• Tissue specificity: BCL2 is expressed in a variety of tissues.
• Keywords associated with Bcl-2 include apoptosis, autophagy, phosphoprotein, proto-oncogene, and disease variant.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser70 of human Bcl-2. For applications like WB and IF/ICC, consider validating specificity by comparing signals from phosphatase-treated and untreated samples.
• When optimizing for ELISA, ensure that the coated antigen or capture/detection format preserves the phosphorylation epitope; using a phospho-specific peptide may help.
• The antibody shows cross reactivity with human and rat Bcl-2; additional species validation may be required if other models are used.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers, labs, and research institutes a seamless procurement experience for IVD raw materials, from concept to clinic. Our catalog includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For Bcl-2-related apoptosis and autophagy research, we can also assist in sourcing rare target raw materials to accelerate your assay development.